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Literature summary for 3.2.1.102 extracted from

  • Higgins, M.A.; Whitworth, G.E.; El Warry, N.; Randriantsoa, M.; Samain, E.; Burke, R.D.; Vocadlo, D.J.; Boraston, A.B.
    Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases (2009), J. Biol. Chem., 284, 26161-26173.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.102

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 17% (v/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, and 0.1 M sodium acetate trihydrate, pH 4.8 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 blood group A glycoconjugate at 37°C Streptococcus pneumoniae
0.57
-
 blood group B glycoconjugate at 37°C Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae SP3-BS71
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
blood group A glycoconjugate + H2O Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen Streptococcus pneumoniae ?
-
 ?
blood group A glycoconjugate + H2O Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen Streptococcus pneumoniae SP3-BS71 ?
-
 ?
blood group B glycoconjugate + H2O Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen Streptococcus pneumoniae ?
-
 ?
blood group B glycoconjugate + H2O Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen Streptococcus pneumoniae SP3-BS71 ?
-
 ?
LewisY tetrasaccharide + H2O Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen Streptococcus pneumoniae ?
-
 ?
LewisY tetrasaccharide + H2O Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen Streptococcus pneumoniae SP3-BS71 ?
-
 ?
additional information Sp3GH98 does not cleave the LewisY tetrasaccharide Streptococcus pneumoniae ?
-
 ?
additional information Sp3GH98 does not cleave the LewisY tetrasaccharide Streptococcus pneumoniae SP3-BS71 ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-galactosidase
-
 Streptococcus pneumoniae
Sp3GH98
-
 Streptococcus pneumoniae
Sp4GH98
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.67
-
 blood group A glycoconjugate at 37°C Streptococcus pneumoniae
11.17
-
 blood group B glycoconjugate at 37°C Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.43
-
 LewisY tetrasaccharide at 37°C Streptococcus pneumoniae
10.67
-
 blood group A glycoconjugate at 37°C Streptococcus pneumoniae
20
-
 blood group B glycoconjugate at 37°C Streptococcus pneumoniae