Cloned (Comment) | Organism |
---|---|
gene eabC, DNA and amino acid sequence determination and analysis, functional overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q6RUF5 | strain 13, gene eabC | - |
Clostridium perfringens ATCC 10543 | Q6RUF5 | strain 13, gene eabC | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain ATCC 10543, by gel filtration, ion exchange, adsorption, and concanavalin A affinity chromatography, and another step of ion exchange chromatography, 2630fold to homogeneity, recombinant His-tagged enzyme by nickel affinity chromatography | Clostridium perfringens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2894 | - |
purified native enzyme | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
blood group A glycoconjugate + H2O | substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR | Clostridium perfringens | ? | - |
? | |
blood group A glycoconjugate + H2O | substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR | Clostridium perfringens ATCC 10543 | ? | - |
? | |
blood group B glycoconjugate + H2O | substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR | Clostridium perfringens | ? | - |
? | |
blood group B glycoconjugate + H2O | substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR | Clostridium perfringens ATCC 10543 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
blood group A- and B-cleaving endo-beta-galactosidase | - |
Clostridium perfringens |
E-ABase | - |
Clostridium perfringens |
More | the enzyme belongs to the GH98 family | Clostridium perfringens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Clostridium perfringens |