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Literature summary for 3.2.1.102 extracted from

  • Kobata, A.; Takasaki, S.
    endo-beta-Galactosidase and endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae (1978), Methods Enzymol., 50, 560-584.
    View publication on PubMed
Search for more literature for 3.2.1.102

Inhibitors

Inhibitors Comment Organism Structure
Ag2SO4 3 mM, 82% inhibition Streptococcus pneumoniae
Cys 3.3 mM, 15 min at 37°C, 52% inhibition Streptococcus pneumoniae
HgCl2 3 mM, 98.6% inhibition Streptococcus pneumoniae
PCMB 3.3 mM, 15 min at 37°C, 88% inhibition Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
0.13
-
 GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc
-
 Streptococcus pneumoniae
0.36
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae
0.67
-
 Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
 Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus pneumoniae

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Streptococcus pneumoniae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.162
-
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glc + H2O
-
 Streptococcus pneumoniae ?
-
 ?
GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
-
 Streptococcus pneumoniae ?
-
 ?
additional information acts specifically on the oligosaccharides with blood group A and B antigenic determinants composed of type 2 chains: the A determinant present in erythrocyte glycoproteins is completely hydrolyzed Streptococcus pneumoniae ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptococcus pneumoniae