BRENDA - Enzyme Database show
show all sequences of 3.2.1.101

An endo-alpha1 -> 6-D-mannanase from a soil bacterium. Purification, properties, and mode of action

Nakajima, T.; Maitra, S.K.; Ballou, C.E.; J. Biol. Chem. 251, 174-181 (1976)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
1% v/v ethanol: slight activation
Bacillus circulans
iodoacetamide
1 mM: slight activation
Bacillus circulans
N-ethylmaleimide
1 mM: slight activation
Bacillus circulans
Propanol
slight activation
Bacillus circulans
Application
Application
Commentary
Organism
analysis
used to determine the structure of mannan-protein linkage region
Bacillus circulans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Bacillus circulans
diisopropyl fluorophosphate
-
Bacillus circulans
EDTA
70% inhibition, 90% of original activity restored with 10 mM Ca2+
Bacillus circulans
ethanol
10% ethanol: inactivation
Bacillus circulans
Fe2+
-
Bacillus circulans
iodoacetamide
2 mM: 50% inhibition
Bacillus circulans
Mannan
Saccharomyces cerevisiae wild type mannan, 20% inhibition
Bacillus circulans
Mg2+
very slight inhibition
Bacillus circulans
N-ethylmaleimide
2 mM: 50% inhibition
Bacillus circulans
p-chloromercuribenzoate
1 mM: complete inhibition, 0.001 mM: 50% inhibition
Bacillus circulans
reduced alpha-1,6-mannohexaose
54% inhibition, also serves as a substrate
Bacillus circulans
reduced alpha-1,6-mannotriose
competitive inhibitor, 92% inhibition
Bacillus circulans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Bacillus circulans
0.0044
-
alpha-1,6-D-mannan
-
Bacillus circulans
0.072
-
alpha-1,6-D-mannohexaose
-
Bacillus circulans
0.12
-
alpha-1,6-D-mannopentaose
-
Bacillus circulans
0.33
-
alpha-1,6-D-mannotetraose
-
Bacillus circulans
1
-
alpha-1,6-D-mannotriose
-
Bacillus circulans
4
-
alpha-1,6-D-mannobiose
-
Bacillus circulans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus circulans
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, required for full activity
Bacillus circulans
Co2+
very slight activation
Bacillus circulans
Mn2+
activation
Bacillus circulans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
gel filtration and SDS-PAGE
Bacillus circulans
131000
-
sedimentation equilibrium
Bacillus circulans
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Ethanol
1% v/v ethanol: stable and slight activation, effect decreases at higher concentrations, at 10% ethanol: inactivation
Bacillus circulans
propanol
stable and slight activation
Bacillus circulans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus circulans
-
Bacillus sp. TN-31: probably Bacillus circulans
-
Purification (Commentary)
Commentary
Organism
-
Bacillus circulans
Reaction
Reaction
Commentary
Organism
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
enzyme hydrolyzes alpha-1,6-D-mannosidic and not beta-1,6-D-mannosidic linkages
Bacillus circulans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Bacillus circulans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.87
-
-
Bacillus circulans
Storage Stability
Storage Stability
Organism
4°C, 0.1 M citrate-phosphate buffer, pH 6.0, or 0.1 M potassium phosphate buffer, pH 6.8, 1 mM CaCl2, stable for at least 1 month
Bacillus circulans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-1,6-D-mannan + H2O
unbranched, with more than 80% alpha-1,6-D-mannosidic linkages, best substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannan + H2O
Saccharomyces cerevisiae X2180-1A backbone mannan
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannan + H2O
Saccharomyces cerevisiae X2180-1A-5 mannan
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannohexaose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannooctaose + H2O
largest substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannooligosaccharide + H2O
smallest substrate: alpha-1,6-mannotriose, largest substrate: alpha-1,6-mannooctaose
135707
Bacillus circulans
mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannopentaose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannotetraose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannotriose + H2O
smallest substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
-
135707
Bacillus circulans
?
additional information
substrate specificity
135707
Bacillus circulans
?
-
-
-
-
additional information
enzyme hydrolyzes alpha-1,6-D-mannosidic and not beta-1,6-D-mannosidic linkages
135707
Bacillus circulans
?
-
-
-
-
additional information
no activity on branched alpha-mannan, alpha-1,6-mannans that contain side chains, little or no activity with glucans: amylose, dextran, cellulose, laminarin, alpha-1,6-mannobiose, reduced mannobiose, reduced mannotriose, no activity with methyl alpha-D-mannoside or p-nitrophenyl alpha-D-mannoside, no activity with beta-1,4-D-mannan, methyl alpha-D-mannopyranoside or p-nitrophenyl alpha-D-mannopyranoside
135707
Bacillus circulans
?
-
-
-
-
reduced mannohexaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannooctaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannopentaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannotetraose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 125000, SDS-PAGE
Bacillus circulans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
pH 5.5
Bacillus circulans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
unusually heat-stable
Bacillus circulans
55
-
stable at, in the presence of substrate, for at least 3 h
Bacillus circulans
60
65
5 min, denatured
Bacillus circulans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.33
-
alpha-1,6-D-mannan
-
Bacillus circulans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
-
Bacillus circulans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
7.5
-
Bacillus circulans
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
p-chloromercuribenzoate
-
Bacillus circulans
2
-
iodoacetamide
-
Bacillus circulans
2
-
N-ethylmaleimide
-
Bacillus circulans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
1% v/v ethanol: slight activation
Bacillus circulans
iodoacetamide
1 mM: slight activation
Bacillus circulans
N-ethylmaleimide
1 mM: slight activation
Bacillus circulans
Propanol
slight activation
Bacillus circulans
Application (protein specific)
Application
Commentary
Organism
analysis
used to determine the structure of mannan-protein linkage region
Bacillus circulans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Bacillus circulans
diisopropyl fluorophosphate
-
Bacillus circulans
EDTA
70% inhibition, 90% of original activity restored with 10 mM Ca2+
Bacillus circulans
ethanol
10% ethanol: inactivation
Bacillus circulans
Fe2+
-
Bacillus circulans
iodoacetamide
2 mM: 50% inhibition
Bacillus circulans
Mannan
Saccharomyces cerevisiae wild type mannan, 20% inhibition
Bacillus circulans
Mg2+
very slight inhibition
Bacillus circulans
N-ethylmaleimide
2 mM: 50% inhibition
Bacillus circulans
p-chloromercuribenzoate
1 mM: complete inhibition, 0.001 mM: 50% inhibition
Bacillus circulans
reduced alpha-1,6-mannohexaose
54% inhibition, also serves as a substrate
Bacillus circulans
reduced alpha-1,6-mannotriose
competitive inhibitor, 92% inhibition
Bacillus circulans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
p-chloromercuribenzoate
-
Bacillus circulans
2
-
iodoacetamide
-
Bacillus circulans
2
-
N-ethylmaleimide
-
Bacillus circulans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Bacillus circulans
0.0044
-
alpha-1,6-D-mannan
-
Bacillus circulans
0.072
-
alpha-1,6-D-mannohexaose
-
Bacillus circulans
0.12
-
alpha-1,6-D-mannopentaose
-
Bacillus circulans
0.33
-
alpha-1,6-D-mannotetraose
-
Bacillus circulans
1
-
alpha-1,6-D-mannotriose
-
Bacillus circulans
4
-
alpha-1,6-D-mannobiose
-
Bacillus circulans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus circulans
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, required for full activity
Bacillus circulans
Co2+
very slight activation
Bacillus circulans
Mn2+
activation
Bacillus circulans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
gel filtration and SDS-PAGE
Bacillus circulans
131000
-
sedimentation equilibrium
Bacillus circulans
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Ethanol
1% v/v ethanol: stable and slight activation, effect decreases at higher concentrations, at 10% ethanol: inactivation
Bacillus circulans
propanol
stable and slight activation
Bacillus circulans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus circulans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Bacillus circulans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.87
-
-
Bacillus circulans
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.1 M citrate-phosphate buffer, pH 6.0, or 0.1 M potassium phosphate buffer, pH 6.8, 1 mM CaCl2, stable for at least 1 month
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-1,6-D-mannan + H2O
unbranched, with more than 80% alpha-1,6-D-mannosidic linkages, best substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannan + H2O
Saccharomyces cerevisiae X2180-1A backbone mannan
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannan + H2O
Saccharomyces cerevisiae X2180-1A-5 mannan
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes
135707
Bacillus circulans
?
alpha-1,6-D-mannohexaose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannooctaose + H2O
largest substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannooligosaccharide + H2O
smallest substrate: alpha-1,6-mannotriose, largest substrate: alpha-1,6-mannooctaose
135707
Bacillus circulans
mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannopentaose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannotetraose + H2O
-
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products
135707
Bacillus circulans
?
alpha-1,6-D-mannotriose + H2O
smallest substrate
135707
Bacillus circulans
D-mannose + alpha-1,6-D-mannobiose
-
135707
Bacillus circulans
?
additional information
substrate specificity
135707
Bacillus circulans
?
-
-
-
-
additional information
enzyme hydrolyzes alpha-1,6-D-mannosidic and not beta-1,6-D-mannosidic linkages
135707
Bacillus circulans
?
-
-
-
-
additional information
no activity on branched alpha-mannan, alpha-1,6-mannans that contain side chains, little or no activity with glucans: amylose, dextran, cellulose, laminarin, alpha-1,6-mannobiose, reduced mannobiose, reduced mannotriose, no activity with methyl alpha-D-mannoside or p-nitrophenyl alpha-D-mannoside, no activity with beta-1,4-D-mannan, methyl alpha-D-mannopyranoside or p-nitrophenyl alpha-D-mannopyranoside
135707
Bacillus circulans
?
-
-
-
-
reduced mannohexaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannooctaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannopentaose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
reduced mannotetraose + H2O
poor substrate
135707
Bacillus circulans
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 125000, SDS-PAGE
Bacillus circulans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
pH 5.5
Bacillus circulans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
unusually heat-stable
Bacillus circulans
55
-
stable at, in the presence of substrate, for at least 3 h
Bacillus circulans
60
65
5 min, denatured
Bacillus circulans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.33
-
alpha-1,6-D-mannan
-
Bacillus circulans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
-
Bacillus circulans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
7.5
-
Bacillus circulans
Other publictions for EC 3.2.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750299
Belz
An atypical interaction expla ...
Bacillus circulans
Chem. Commun. (Camb.)
53
9238-9241
2017
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135708
Brigance
Organization of the yeast golg ...
Bacillus circulans
Mol. Biol. Cell
11
171-182
2000
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650611
Maruyama
The aman6 gene encoding a yeas ...
Bacillus circulans
Biosci. Biotechnol. Biochem.
64
2018-2020
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
135707
Nakajima
An endo-alpha1 -> 6-D-mannanas ...
Bacillus circulans
J. Biol. Chem.
251
174-181
1976
4
1
-
-
-
-
12
7
1
3
2
-
2
1
-
-
1
1
-
1
1
1
16
1
1
-
3
1
1
1
-
-
3
-
-
4
1
-
-
-
-
-
-
12
3
7
1
3
2
-
2
-
-
1
-
1
1
1
16
1
1
-
3
1
1
1
-
-
-
-
-
-
-
-
135709
Nakajima
Structure of the linkage regio ...
Bacillus circulans
J. Biol. Chem.
249
7685-7694
1974
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
3
2
-
1
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-