Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 1% v/v ethanol: slight activation | Niallia circulans | |
iodoacetamide | 1 mM: slight activation | Niallia circulans | |
N-ethylmaleimide | 1 mM: slight activation | Niallia circulans | |
Propanol | slight activation | Niallia circulans |
Application | Comment | Organism |
---|---|---|
analysis | used to determine the structure of mannan-protein linkage region | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Niallia circulans | |
diisopropyl fluorophosphate | - |
Niallia circulans | |
EDTA | 70% inhibition, 90% of original activity restored with 10 mM Ca2+ | Niallia circulans | |
ethanol | 10% ethanol: inactivation | Niallia circulans | |
Fe2+ | - |
Niallia circulans | |
iodoacetamide | 2 mM: 50% inhibition | Niallia circulans | |
Mannan | Saccharomyces cerevisiae wild type mannan, 20% inhibition | Niallia circulans | |
Mg2+ | very slight inhibition | Niallia circulans | |
N-ethylmaleimide | 2 mM: 50% inhibition | Niallia circulans | |
p-chloromercuribenzoate | 1 mM: complete inhibition, 0.001 mM: 50% inhibition | Niallia circulans | |
reduced alpha-1,6-mannohexaose | 54% inhibition, also serves as a substrate | Niallia circulans | |
reduced alpha-1,6-mannotriose | competitive inhibitor, 92% inhibition | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Niallia circulans | |
0.0044 | - |
alpha-1,6-D-mannan | - |
Niallia circulans | |
0.072 | - |
alpha-1,6-D-mannohexaose | - |
Niallia circulans | |
0.12 | - |
alpha-1,6-D-mannopentaose | - |
Niallia circulans | |
0.33 | - |
alpha-1,6-D-mannotetraose | - |
Niallia circulans | |
1 | - |
alpha-1,6-D-mannotriose | - |
Niallia circulans | |
4 | - |
alpha-1,6-D-mannobiose | - |
Niallia circulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Niallia circulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, required for full activity | Niallia circulans | |
Co2+ | very slight activation | Niallia circulans | |
Mn2+ | activation | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
gel filtration and SDS-PAGE | Niallia circulans |
131000 | - |
sedimentation equilibrium | Niallia circulans |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 1% v/v ethanol: stable and slight activation, effect decreases at higher concentrations, at 10% ethanol: inactivation | Niallia circulans |
propanol | stable and slight activation | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | - |
Bacillus sp. TN-31: probably Bacillus circulans | - |
Purification (Comment) | Organism |
---|---|
- |
Niallia circulans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(1,6-beta-D-mannopyranosyl)n-1,6-beta-D-mannopyranose + H2O = (1,6-beta-D-mannopyranosyl)n-2-1,6-beta-D-mannopyranose + 6-O-beta-D-mannopyranosyl-beta-D-mannopyranose | enzyme hydrolyzes alpha-1,6-D-mannosidic and not beta-1,6-D-mannosidic linkages | Niallia circulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Niallia circulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.87 | - |
- |
Niallia circulans |
Storage Stability | Organism |
---|---|
4°C, 0.1 M citrate-phosphate buffer, pH 6.0, or 0.1 M potassium phosphate buffer, pH 6.8, 1 mM CaCl2, stable for at least 1 month | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,6-D-mannan + H2O | unbranched, with more than 80% alpha-1,6-D-mannosidic linkages, best substrate | Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes | ? | |
alpha-1,6-D-mannan + H2O | Saccharomyces cerevisiae X2180-1A backbone mannan | Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes | ? | |
alpha-1,6-D-mannan + H2O | Saccharomyces cerevisiae X2180-1A-5 mannan | Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of alpha-1,6-mannooligosaccharides of various sizes | ? | |
alpha-1,6-D-mannohexaose + H2O | - |
Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products | ? | |
alpha-1,6-D-mannooctaose + H2O | largest substrate | Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products | ? | |
alpha-1,6-D-mannooligosaccharide + H2O | smallest substrate: alpha-1,6-mannotriose, largest substrate: alpha-1,6-mannooctaose | Niallia circulans | mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products | ? | |
alpha-1,6-D-mannopentaose + H2O | - |
Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products | ? | |
alpha-1,6-D-mannotetraose + H2O | - |
Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | end-product, with intermediate formation of mannose, mannobiose and mannotriose, the latter apparently converts more slowly to the smaller products | ? | |
alpha-1,6-D-mannotriose + H2O | smallest substrate | Niallia circulans | D-mannose + alpha-1,6-D-mannobiose | - |
? | |
additional information | substrate specificity | Niallia circulans | ? | - |
? | |
additional information | enzyme hydrolyzes alpha-1,6-D-mannosidic and not beta-1,6-D-mannosidic linkages | Niallia circulans | ? | - |
? | |
additional information | no activity on branched alpha-mannan, alpha-1,6-mannans that contain side chains, little or no activity with glucans: amylose, dextran, cellulose, laminarin, alpha-1,6-mannobiose, reduced mannobiose, reduced mannotriose, no activity with methyl alpha-D-mannoside or p-nitrophenyl alpha-D-mannoside, no activity with beta-1,4-D-mannan, methyl alpha-D-mannopyranoside or p-nitrophenyl alpha-D-mannopyranoside | Niallia circulans | ? | - |
? | |
reduced mannohexaose + H2O | poor substrate | Niallia circulans | ? | - |
? | |
reduced mannooctaose + H2O | poor substrate | Niallia circulans | ? | - |
? | |
reduced mannopentaose + H2O | poor substrate | Niallia circulans | ? | - |
? | |
reduced mannotetraose + H2O | poor substrate | Niallia circulans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 125000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.5 | Niallia circulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
unusually heat-stable | Niallia circulans |
55 | - |
stable at, in the presence of substrate, for at least 3 h | Niallia circulans |
60 | 65 | 5 min, denatured | Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.33 | - |
alpha-1,6-D-mannan | - |
Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Niallia circulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Niallia circulans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
p-chloromercuribenzoate | - |
Niallia circulans | |
2 | - |
N-ethylmaleimide | - |
Niallia circulans | |
2 | - |
iodoacetamide | - |
Niallia circulans |