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Literature summary for 3.2.1.10 extracted from

  • Deng, X.; Petitjean, M.; Teste, M.; Kooli, W.; Tranier, S.; Francois, J.; Parrou, J.
    Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae (2014), FEBS Open Bio, 4, 200-212.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.10

Protein Variants

Protein Variants Comment Organism
L240P similar to wild-type Saccharomyces cerevisiae
R279Q displays substrate specificities and specific activities close to those of isoform Ima2 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl alpha-D-glucopyranoside
-
 Saccharomyces cerevisiae
isomaltose
-
 Saccharomyces cerevisiae
maltose competitive; competitive; mixed Saccharomyces cerevisiae
methyl alpha-D-glucopyranoside
-
 Saccharomyces cerevisiae
palatinose
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
0.48
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
0.58
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
0.89
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
7
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
11
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
12
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
13
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
15
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
17
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
18
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
27
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
28
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
37
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
72
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
83
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
116
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
128
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
144
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
147
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
191
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P0CW40 isoform Ima3
-
Saccharomyces cerevisiae P40884 isoform Ima5
-
Saccharomyces cerevisiae P53051 isoform Ima1
-
Saccharomyces cerevisiae Q08295 isoform Ima2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
3.9
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
5
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
5.8
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
7.2
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
7.6
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
7.7
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
7.9
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
8.6
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
10
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
10.2
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
11
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
18.6
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
19.7
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
39
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
52
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
52
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
54
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
55
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
60
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
65
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
74
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O
-
 Saccharomyces cerevisiae 2 D-glucose
-
 ?
isomaltriose + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
methyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae methanol + alpha-D-glucopyranose
-
 ?
additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis-Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ?
-
 ?
additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis–Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ?
-
 ?
palatinose + H2O
-
 Saccharomyces cerevisiae D-glucose + D-fructose
-
 ?
sucrose + H2O
-
 Saccharomyces cerevisiae D-glucose + D-fructose
-
 ?

Synonyms

Synonyms Comment Organism
Ima2
-
 Saccharomyces cerevisiae
Ima3
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
 Saccharomyces cerevisiae
43
-
-
 Saccharomyces cerevisiae
46
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
29.9
-
 melting temperature, pH 8 Saccharomyces cerevisiae
33.2
-
 melting temperature, pH 8 Saccharomyces cerevisiae
35.7
-
 melting temperature, pH 8 Saccharomyces cerevisiae
36.3
-
 melting temperature, pH 7 Saccharomyces cerevisiae
36.6
-
 melting temperature, pH 6 Saccharomyces cerevisiae
37
-
 half-life 2.9 min Saccharomyces cerevisiae
37
-
 half-life 23.2 min Saccharomyces cerevisiae
37
-
 half-life 26.1 min Saccharomyces cerevisiae
37
-
 half-life 27.4 min Saccharomyces cerevisiae
38.8
-
 melting temperature, pH 7 Saccharomyces cerevisiae
40
-
 half-life 7.6 min Saccharomyces cerevisiae
40
-
 half-life 17.6 min Saccharomyces cerevisiae
40
-
 half-life 0.8 min Saccharomyces cerevisiae
40
-
 half-life 18.5 min Saccharomyces cerevisiae
41.6
-
 melting temperature, pH 8 Saccharomyces cerevisiae
46.2
-
 melting temperature, pH 7 Saccharomyces cerevisiae
46.6
-
 melting temperature, pH 7 Saccharomyces cerevisiae
47
-
 melting temperature, pH 6 Saccharomyces cerevisiae
55.1
-
 melting temperature, pH 6 Saccharomyces cerevisiae
55.5
-
 melting temperature, pH 6 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
6
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
7.1
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
8.7
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
14
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
14
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
15
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
15
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
18
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
21
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
25
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
51
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
55
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
56
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
78
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
84
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
88
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
97
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
101
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
103
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
104
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharomyces cerevisiae
7.6
-
-
 Saccharomyces cerevisiae
8
-
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2 5 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
9.8
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
12
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
188
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
246
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
248
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
574
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
600
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
686
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
690
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
0.16
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
0.21
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
0.22
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
0.22
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
0.35
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
0.38
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
0.41
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
0.8
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
0.9
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.1
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
4.3
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
5
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
5.6
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
5.7
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
8
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
9.4
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
15
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
16
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
20
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
28
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae