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Literature summary for 3.2.1.10 extracted from
- Substrate recognition by three family 13 yeast alpha-glucosidases. Evaluation of deoxygenated and conformationally biased isomaltosides (2002), Eur. J. Biochem., 269, 728-734.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, transition state stabilization energy | Saccharomyces cerevisiae | |
| 1.3 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C | Saccharomyces cerevisiae |  |
| 6.9 | - |
isomaltose | pH 6.8, 30°C | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose | stereochemistry, molecular substrate recognition mechanism, catayltic mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
catalytic efficiency with different substrates, substrate specificity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 2-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3'-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 4'-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 4-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 4-nitrophenyl alpha-D-glucopyranoside | - |
Saccharomyces cerevisiae | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| 6'-deoxy-methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 6(R)-ethyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 6(S)-ethyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| isomaltose + H2O | - |
Saccharomyces cerevisiae | alpha-D-glucose + D-glucose | - |
? | |
| methyl-alpha-isomaltoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | stereochemistry and -specificity, overview | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dextrin 6-alpha-glucanohydrolase | - |
Saccharomyces cerevisiae |
| More | enzyme belongs to alpha-glucoside hydrolase family 13 | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 33.3 | - |
isomaltose | pH 6.8, 30°C | Saccharomyces cerevisiae | |
| 129 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.8, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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