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Literature summary for 3.2.1.10 extracted from
- Oligo-1,6-glucosidase from a thermophile, Bacillus thermoglucosidasius KP1006, was efficiently produced by combinatorial expression of GroEL in Escherichia coli (2002), Biotechnol. Appl. Biochem., 35, 35-43.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GroEL | heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.56fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli | Parageobacillus thermoglucosidasius | |
| GroES | heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.44fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli | Parageobacillus thermoglucosidasius |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| optimization of recombinant overproduction in Escherichia coli BL21(DE3) by coexpression with various Bacillus thermoglucosidarius chaperone proteins, i.e. Hsp60 team proteins GroES and GroEL, as well as Hsp70 team proteins GrpE, DnaK, and DnaJ, thereof the Hsp70 proteins have no effect on expression level, while the Hsp60 proteins, especially GroEL, increase the expression level by 3.8fold | Parageobacillus thermoglucosidasius |
General Stability
| General Stability | Organism |
|---|---|
| N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization | Parageobacillus thermoglucosidasius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DnaJ | the coexpression of the Hsp70 team chaperone protein leads to reduced activity | Parageobacillus thermoglucosidasius | |
| DnaK | the coexpression of the Hsp70 team chaperone protein leads to reduced activity | Parageobacillus thermoglucosidasius | |
| GrpE | the coexpression of the Hsp70 team chaperone protein leads to reduced activity | Parageobacillus thermoglucosidasius |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000 | Parageobacillus thermoglucosidasius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parageobacillus thermoglucosidasius | - |
- |
- |
| Parageobacillus thermoglucosidasius KP1006 | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| thermally inactivated enzyme is revived by GroEL | Parageobacillus thermoglucosidasius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Parageobacillus thermoglucosidasius |
| 5.1 | - |
purified recombinant enzyme + coexpressed DnaJ, DnaK, and GrpE | Parageobacillus thermoglucosidasius |
| 6.07 | - |
purified recombinant enzyme | Parageobacillus thermoglucosidasius |
| 8.74 | - |
purified recombinant enzyme + coexpressed GroES | Parageobacillus thermoglucosidasius |
| 9.46 | - |
purified recombinant enzyme + coexpressed GroES and GroEL | Parageobacillus thermoglucosidasius |
| 9.48 | - |
purified recombinant enzyme + coexpressed GroEL | Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Parageobacillus thermoglucosidasius | 4-nitrophenol + alpha-D-glucopyranose | - |
? |  |
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Parageobacillus thermoglucosidasius KP1006 | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| additional information | N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization | Parageobacillus thermoglucosidasius | ? | - |
? | |
| additional information | N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization | Parageobacillus thermoglucosidasius KP1006 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000 | Parageobacillus thermoglucosidasius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycosyl hydrolase family 13 | Parageobacillus thermoglucosidasius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Parageobacillus thermoglucosidasius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Parageobacillus thermoglucosidasius |
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