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Literature summary for 3.2.1.10 extracted from

  • Yamamoto, M.; Horikoshi, K.
    Purification and properties of an oligo-1,6-glucosidase from alkalophilic Bacillus species (1990), Carbohydr. Res., 197, 227-235.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition by 1 mM CuCl2 Bacillus sp. F5
Fe2+ 90% inhibition by 1 mM FeCl2 Bacillus sp. F5
Hg2+ complete inhibition by 1 mM HgCl2 Bacillus sp. F5
monoiodoacetate 77% inhibition by 1 mM Bacillus sp. F5
p-chloromercuribenzoate complete inhibition by 0.1 mM Bacillus sp. F5
Pb2+ complete inhibition by 1 mM PbCl2 Bacillus sp. F5
Zn2+ 99% inhibition by 1 mM ZnCl2 Bacillus sp. F5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
panose
-
Bacillus sp. F5
1
-
Panitol
-
Bacillus sp. F5
1.7
-
isomaltotriose
-
Bacillus sp. F5
1.9
-
maltotriose
-
Bacillus sp. F5
3.33
-
isomaltose
-
Bacillus sp. F5
4
-
maltotriitol
-
Bacillus sp. F5
13.3
-
maltose
-
Bacillus sp. F5

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 62000 SDS-PAGE Bacillus sp. F5
63000
-
Sepharyl S-200 gel filtration Bacillus sp. F5

Organism

Organism UniProt Comment Textmining
Bacillus sp. F5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. F5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.2
-
purified enzyme, isomaltose as substrate Bacillus sp. F5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltosaccharides + H2O n = 2-6 Bacillus sp. F5 D-glucose
-
?
isomaltose + H2O
-
Bacillus sp. F5 2 D-glucose
-
?
isomaltotriose + H2O
-
Bacillus sp. F5 3 alpha-D-glucose
-
?
maltose + H2O
-
Bacillus sp. F5 2 D-glucose
-
?
maltotriitol + H2O
-
Bacillus sp. F5 D-glucose + maltitol
-
?
maltotriose + H2O
-
Bacillus sp. F5 maltose + D-glucose
-
?
panitol
-
Bacillus sp. F5 glucitol + maltitol
-
?
panose + H2O
-
Bacillus sp. F5 D-glucose + maltose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus sp. F5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 stable up to 50°C, no activity at 60°C Bacillus sp. F5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 at 45°C, isomaltose as substrate Bacillus sp. F5

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 at 50°C, 10 min incubation Bacillus sp. F5