Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition by 1 mM CuCl2 | Bacillus sp. F5 | |
Fe2+ | 90% inhibition by 1 mM FeCl2 | Bacillus sp. F5 | |
Hg2+ | complete inhibition by 1 mM HgCl2 | Bacillus sp. F5 | |
monoiodoacetate | 77% inhibition by 1 mM | Bacillus sp. F5 | |
p-chloromercuribenzoate | complete inhibition by 0.1 mM | Bacillus sp. F5 | |
Pb2+ | complete inhibition by 1 mM PbCl2 | Bacillus sp. F5 | |
Zn2+ | 99% inhibition by 1 mM ZnCl2 | Bacillus sp. F5 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
panose | - |
Bacillus sp. F5 | |
1 | - |
Panitol | - |
Bacillus sp. F5 | |
1.7 | - |
isomaltotriose | - |
Bacillus sp. F5 | |
1.9 | - |
maltotriose | - |
Bacillus sp. F5 | |
3.33 | - |
isomaltose | - |
Bacillus sp. F5 | |
4 | - |
maltotriitol | - |
Bacillus sp. F5 | |
13.3 | - |
maltose | - |
Bacillus sp. F5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 62000 | SDS-PAGE | Bacillus sp. F5 |
63000 | - |
Sepharyl S-200 gel filtration | Bacillus sp. F5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. F5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. F5 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.2 | - |
purified enzyme, isomaltose as substrate | Bacillus sp. F5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltosaccharides + H2O | n = 2-6 | Bacillus sp. F5 | D-glucose | - |
? | |
isomaltose + H2O | - |
Bacillus sp. F5 | 2 D-glucose | - |
? | |
isomaltotriose + H2O | - |
Bacillus sp. F5 | 3 alpha-D-glucose | - |
? | |
maltose + H2O | - |
Bacillus sp. F5 | 2 D-glucose | - |
? | |
maltotriitol + H2O | - |
Bacillus sp. F5 | D-glucose + maltitol | - |
? | |
maltotriose + H2O | - |
Bacillus sp. F5 | maltose + D-glucose | - |
? | |
panitol | - |
Bacillus sp. F5 | glucitol + maltitol | - |
? | |
panose + H2O | - |
Bacillus sp. F5 | D-glucose + maltose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. F5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | stable up to 50°C, no activity at 60°C | Bacillus sp. F5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | at 45°C, isomaltose as substrate | Bacillus sp. F5 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | at 50°C, 10 min incubation | Bacillus sp. F5 |