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Literature summary for 3.2.1.10 extracted from
- Purification and characterization of Bacillus coagulans oligo-1,6-glucosidase (1986), Eur. J. Biochem., 158, 77-83.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Bacillus cereus |  |
| Ba2+ | 72% inhibition by 2 mM BaCl2 | Weizmannia coagulans | |
| Ca2+ | 29% inhibition by 2 mM CaCl2 | Weizmannia coagulans | |
| Cd2+ | complete inhibition | Weizmannia coagulans | |
| Co2+ | - |
Bacillus cereus | |
| Co2+ | complete inhibition | Weizmannia coagulans | |
| Cu2+ | - |
Bacillus cereus | |
| Cu2+ | 96% inhibition by 2 mM CuCl2 | Weizmannia coagulans | |
| D-glucose | - |
Bacillus cereus | |
| D-glucose | 52% inhibition by 10 mM, competitive | Weizmannia coagulans | |
| EDTA | no inhibition by 2 mM at pH 7.5 | Bacillus cereus | |
| EDTA | no inhibition by 2 mM at pH 7.5 | Weizmannia coagulans | |
| Fe3+ | 86% inhibition by 2 mM FeCl3 | Weizmannia coagulans | |
| glucono-delta-lactone | - |
Bacillus cereus | |
| glucono-delta-lactone | 57% inhibition by 10 mM, competitive | Weizmannia coagulans | |
| Hg2+ | - |
Bacillus cereus | |
| Hg2+ | 99% inhibition by 2 mM HgCl2 | Weizmannia coagulans | |
| Mg2+ | 42% inhibition by 2 mM MgCl2 | Weizmannia coagulans | |
| Mn2+ | 92% inhibition by 2 mM MbCl2 | Weizmannia coagulans | |
| Ni2+ | 94% inhibition by 2 mM NiCl2 | Weizmannia coagulans | |
| p-nitrophenol | - |
Bacillus cereus | |
| p-nitrophenol | complete inhibition by 2 mM | Weizmannia coagulans | |
| Pb2+ | - |
Bacillus cereus | |
| Pb2+ | complete inhibition | Weizmannia coagulans | |
| phenol | in combination with Tris most potent inhibitor | Weizmannia coagulans | |
| phenyl alpha-D-glucoside | - |
Bacillus cereus | |
| phenyl alpha-D-glucoside | 58% inhibition by 10 mM | Weizmannia coagulans | |
| Phenyl alpha-maltoside | 92% inhibition by 10 mM, noncompetitive | Weizmannia coagulans | |
| Sn2+ | complete inhibition | Weizmannia coagulans | |
| Tris | - |
Bacillus cereus | |
| Tris | 89% inhibition by 5 mM, competitive | Weizmannia coagulans | |
| Urea | - |
Bacillus cereus | |
| Urea | 50% inactivation after treatment with 4.6 M for 5 h at 25°C and pH 6.8 | Weizmannia coagulans | |
| Zn2+ | - |
Bacillus cereus | |
| Zn2+ | complete inhibition | Weizmannia coagulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
p-nitrophenyl alpha-D-glucopyranoside | - |
Weizmannia coagulans | |
| 3 | - |
isomaltose | - |
Weizmannia coagulans | |
| 4.4 | - |
isomaltotriose | - |
Weizmannia coagulans | |
| 7.4 | - |
isomaltotetraose | - |
Weizmannia coagulans | |
| 13 | - |
isomaltopentaose | - |
Weizmannia coagulans | |
| 15 | - |
isomaltohexaose | - |
Weizmannia coagulans | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | - |
Bacillus cereus |
| Ethanol | 50% inactivation after 5 h treatment with 32% ethanol at 25°C and pH 6.8 | Weizmannia coagulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
ATCC 7064 | - |
| Weizmannia coagulans | - |
ATCC 7050 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-limit dextrins + H2O | - |
Bacillus cereus | D-glucose | - |
? | |
| alpha-limit dextrins + H2O | - |
Weizmannia coagulans | D-glucose | - |
? | |
| isomaltohexaose + H2O | - |
Weizmannia coagulans | 6 alpha-D-glucose | - |
? | |
| isomaltopentaose + H2O | - |
Weizmannia coagulans | 5 alpha-D-glucose | - |
? | |
| isomaltosaccharides + H2O | n = 2-6 | Bacillus cereus | D-glucose | - |
? | |
| isomaltosaccharides + H2O | n = 2-6 | Weizmannia coagulans | D-glucose | - |
? | |
| isomaltose + H2O | - |
Weizmannia coagulans | alpha-D-glucose + D-glucose | - |
? | |
| isomaltotetraose + H2O | - |
Weizmannia coagulans | 4 alpha-D-glucose | - |
? | |
| isomaltotriose + H2O | - |
Weizmannia coagulans | 3 alpha-D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Weizmannia coagulans | D-glucose + p-nitrophenol | - |
? | |
| panose + H2O | - |
Bacillus cereus | D-glucose + maltose | - |
? | |
| panose + H2O | - |
Weizmannia coagulans | D-glucose + maltose | - |
? | |
| phenyl-alpha-D-glucopyranoside + H2O | - |
Weizmannia coagulans | D-glucose + phenol | - |
? | |
| sucrose + H2O | - |
Weizmannia coagulans | D-glucose + D-fructose | - |
? | |
| turanose + H2O | - |
Weizmannia coagulans | D-glucose + D-fructose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Weizmannia coagulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | 75 | half-optima at 47°C and 75°C, no activity at 78°C | Weizmannia coagulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
stable at pH 6.8 for 10 min | Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Weizmannia coagulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | 10.2 | half-optima at pH 4.8 and 7.3 | Weizmannia coagulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.1 | 50% inactivation at pH 5.0 and 9.1 after incubation at 34°C for 15 h | Weizmannia coagulans |
| 6.5 | 7.3 | 50% inactivation at pH 6.5 and 7.3 after incubation at 34°C for 15 h | Bacillus cereus |
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