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Literature summary for 3.2.1.10 extracted from
- A strong correlation between the increase in number of proline residues and the rise in thermostability of five Bacillus oligo-1,6-glucosidases (1987), Appl. Microbiol. Biotechnol., 26, 546-551.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | - |
Bacillus cereus |  |
| Urea | 50% inactivation after treatment with 8.7 M for 5 h at 25°C or 2.4 M at 60°C and pH 6.8 | Bacillus thermoamyloliquefaciens KP1071 | |
| Urea | 50% inactivation after treatment with 9.2 M for 5 h at 25°C or 2.7 M at 60°C and pH 6.8 | Parageobacillus thermoglucosidasius | |
| Urea | - |
Weizmannia coagulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | 62000 | - |
Weizmannia coagulans |
| 60000 | 62000 | - |
Parageobacillus thermoglucosidasius |
| 60000 | 62000 | - |
Bacillus flavocaldarius |
| 60000 | 62000 | gel filtration | Bacillus thermoamyloliquefaciens KP1071 |
| 60000 | 62000 | SDS-PAGE | Bacillus cereus |
| 60000 | 62000 | SDS-PAGE | Bacillus thermoamyloliquefaciens KP1071 |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 50% inactivation after 5 h treatment with 32% ethanol at 25°C and pH 6.8 | Weizmannia coagulans |
| Ethanol | 50% inactivation after 5 h treatment with 49% ethanol at 25°C and pH 6.8 | Parageobacillus thermoglucosidasius |
| Ethanol | 50% inactivation after 5 h treatment with 41% ethanol at 25°C and pH 6.8 | Bacillus thermoamyloliquefaciens KP1071 |
| Ethanol | fairly stable for 5 h at 4°C in 20% ethanol , 50% inactivation after 5 h treatment with 17% ethanol at 25°C and pH 6.8 | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
ATCC 7064 | - |
| Bacillus flavocaldarius | - |
- |
- |
| Bacillus thermoamyloliquefaciens KP1071 | - |
- |
- |
| Parageobacillus thermoglucosidasius | - |
- |
- |
| Weizmannia coagulans | - |
ATCC 7050 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus thermoamyloliquefaciens KP1071 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Weizmannia coagulans | D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Parageobacillus thermoglucosidasius | D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus flavocaldarius | D-glucose + p-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus thermoamyloliquefaciens KP1071 | D-glucose + p-nitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dextrin 6-alpha-D-glucanohydrolase | - |
Bacillus cereus |
| dextrin 6-alpha-D-glucanohydrolase | - |
Parageobacillus thermoglucosidasius |
| dextrin 6-alpha-D-glucanohydrolase | - |
Bacillus thermoamyloliquefaciens KP1071 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 74 | - |
half-optima at 58°C and 75°C | Bacillus thermoamyloliquefaciens KP1071 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability rises with proline content | Bacillus cereus |
| additional information | - |
thermostability rises with proline content | Weizmannia coagulans |
| additional information | - |
thermostability rises with proline content | Parageobacillus thermoglucosidasius |
| additional information | - |
thermostability rises with proline content | Bacillus flavocaldarius |
| additional information | - |
thermostability rises with proline content | Bacillus thermoamyloliquefaciens KP1071 |
| 4 | 60 | stable activity for 10 min at pH 6.8, 50% inactivation after 10 min at 70°C | Bacillus thermoamyloliquefaciens KP1071 |
| 25 | 55 | stable for 15 h at pH 5.5-10.3 at 25°C, at pH 6.4-10.1 at 34°C, at pH 6.8-8.4 at 55°C, no activity after 15 h at pH 2.8 and 11.4 at 25°C, at pH 3.1 and 11.1 at 34°C, at pH 3.8 and 9.9 at 55°C | Bacillus thermoamyloliquefaciens KP1071 |
| 70 | - |
50% inactivation after 10 min incubation at pH 6.8 | Bacillus thermoamyloliquefaciens KP1071 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
- |
Bacillus thermoamyloliquefaciens KP1071 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 9.8 | half-optima at pH 4.9 and 7.6 | Bacillus thermoamyloliquefaciens KP1071 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 11.3 | 50% inactivation at pH 3.5 and 11.3 after incubation at 34°C for 15 h | Parageobacillus thermoglucosidasius |
| 4.3 | 10.7 | 50% inactivation at pH 4.3 and 10.7 after incubation at 34°C for 15 h | Bacillus thermoamyloliquefaciens KP1071 |
| 5 | 9.1 | 50% inactivation at pH 5.0 and 9.1 after incubation at 34°C for 15 h | Weizmannia coagulans |
| 6.5 | 7.3 | 50% inactivation at pH 6.5 and 7.3 after incubation at 34°C for 15 h | Bacillus cereus |
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