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Literature summary for 3.2.1.10 extracted from

  • Plant, A.R.; Parratt, S.; Daniel, R.M.; Morgan, H.W.
    A cell-associated oligo-1,6-alpha-glucosidase from an extremely thermophilic anaerobic bacterium, Thermoanaerobium Tok6-B1 (1988), Biochem. J., 255, 865-868.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.10

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2.3fold increase of activity by 1 mM Thermoanaerobium sp.
dithiothreitol
-
 Thermoanaerobium sp.

Inhibitors

Inhibitors Comment Organism Structure
1-O-methyl-alpha-D-glucose 42% inhibition by 62 mM Thermoanaerobium sp.
1-O-methyl-beta-D-glucose 52% inhibition by 62 mM Thermoanaerobium sp.
D-glucose product inhibition, inhibition of 70.1% of activity by 110 mM Thermoanaerobium sp.
EDTA rapid loss of activity at 1 mM, pH 5.6, 76°C Thermoanaerobium sp.
N-ethylmaleimide 70% inactivation by 3.3 mM at room temperature after 10 min Thermoanaerobium sp.
SDS 96% inhibition by 3.5 mM under standard conditions Thermoanaerobium sp.
Urea 26% inhibition by 4 mM under standard conditions Thermoanaerobium sp.
Zn2+ rapid inactivation by 1 mM possibly through interaction with free thiol groups of the enzyme Thermoanaerobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 p-nitrophenyl-alpha-D-glucopyranoside
-
 Thermoanaerobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilization of activity by 1 mM at pH 5.6, 76°C Thermoanaerobium sp.
Mg2+ stabilization of activity by 1 mM at pH 5.6, 76°C Thermoanaerobium sp.
Mn2+ stabilization of activity by 1 mM at pH 5.6, 76°C Thermoanaerobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000 33000 SDS-PAGE, gel filtration Thermoanaerobium sp.

Organism

Organism UniProt Comment Textmining
Thermoanaerobium sp.
-
 Tok6-B1
-
Thermoanaerobium sp. Tok6-B1
-
 Tok6-B1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoanaerobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.19
-
 purified enzyme, p-nitrophenyl-alpha-D-glucopyranoside as substrate Thermoanaerobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltosaccharides + H2O n = 2-3 Thermoanaerobium sp. D-glucose
-
 ?
isomaltose + H2O
-
 Thermoanaerobium sp. 2 D-glucose
-
 ?
isomaltose + H2O
-
 Thermoanaerobium sp. Tok6-B1 2 D-glucose
-
 ?
additional information no degradation of p-nitrophenyl-beta-D-glucose, p-nitrophenyl-alpha-D-galactose, p-nitrophenyl-beta-D-galactose, p-nitrophenyl-beta-D-xylose, p-nitrophenyl-alpha-L-arabinose, N-acetyl-alpha-D-glucosamine at 1 mM under standard conditions Thermoanaerobium sp. ?
-
 ?
additional information no degradation of p-nitrophenyl-beta-D-glucose, p-nitrophenyl-alpha-D-galactose, p-nitrophenyl-beta-D-galactose, p-nitrophenyl-beta-D-xylose, p-nitrophenyl-alpha-L-arabinose, N-acetyl-alpha-D-glucosamine at 1 mM under standard conditions Thermoanaerobium sp. Tok6-B1 ?
-
 ?
nigerose + H2O weak activity Thermoanaerobium sp. 2 D-glucose
-
 ?
nigerose + H2O weak activity Thermoanaerobium sp. Tok6-B1 2 D-glucose
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Thermoanaerobium sp. D-glucose + p-nitrophenol
-
 ?
palatinose + H2O weak aktivity Thermoanaerobium sp. D-glucose + fructose
-
 ?
palatinose + H2O weak aktivity Thermoanaerobium sp. Tok6-B1 D-glucose + fructose
-
 ?
panose + H2O
-
 Thermoanaerobium sp. D-glucose + maltose
-
 ?
panose + H2O
-
 Thermoanaerobium sp. Tok6-B1 D-glucose + maltose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
-
 Thermoanaerobium sp.
76
-
 half-life 20 min at pH 6.5 Thermoanaerobium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7
-
 Thermoanaerobium sp.