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Literature summary for 3.2.1.1 extracted from
- Effect of cultivation conditions on activity of alpha-amylase from a tropical strain Aspergillus flavus Link (2018), J. Microbiol. Biotechnol. Food Sci., 7, 571-575 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Aspergillus flavus |  |
| Na+ | - |
Aspergillus flavus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dinitrophenol | - |
Aspergillus flavus | |
| EDTA | - |
Aspergillus flavus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
soluble starch | apparent Km value of 7.1 mg/ml, pH 6, 35°C | Aspergillus flavus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus flavus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, by ammonium sulfate precipitation followed by dialysis | Aspergillus flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 523 | - |
pH 6, 35°C | Aspergillus flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + H2O | - |
Aspergillus flavus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Aspergillus flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
20 min, 64% loss of activity | Aspergillus flavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus flavus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Aspergillus flavus | potassium nitrate, ammonium sulfate and casein, casein as nitrogen source in the defined growth medium with starch as carbon source support the highest activity of alpha-amylase | additional information |
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