Any feedback?
Literature summary for 3.2.1.1 extracted from
- Parameters optimization and kinetics study of alpha-amylase enzyme of Bacillus sp. MB6 isolated from vegetable waste (2017), Process Biochem., 52, 123-129 .
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme immobilization, best at 4% alginate as compared to agar agar and other concentrations of alginate, overview | Bacillus sp. MB6 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, Km is 5.45 mg/ml and Vmax is 24.15 mg/ml/min | Bacillus sp. MB6 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Bacillus sp. MB6 | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. MB6 | - |
isolated from vegetable waste sample, which has been collected from the local vegetable market of Raipur, Chhattisgarh, India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 1.63fold by ammonium sulfate fractionation and dialysis | Bacillus sp. MB6 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimization of the enzyme production method, overview. Best at pH 6.0, 37°C for 48 h | Bacillus sp. MB6 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 270.59 | - |
immobilized enzyme, pH 6.0, 55°C | Bacillus sp. MB6 |
| 402.02 | - |
partially purified enzyme, pH 6.0, 55°C | Bacillus sp. MB6 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Bacillus sp. MB6 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Bacillus sp. MB6 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus sp. MB6 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus sp. MB6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
