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Literature summary for 3.2.1.1 extracted from
- Characterization and application of BiLA, a psychrophilic alpha-amylase from Bifidobacterium longum (2016), J. Agric. Food Chem., 64, 2709-2718 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by 5 mM EDTA | Bifidobacterium longum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bllj_0710, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain MC1061 | Bifidobacterium longum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 60% inhibition at 5 mM | Bifidobacterium longum |  |
| Cu2+ | complete inhibition at 5 mM | Bifidobacterium longum | |
| Fe3+ | complete inhibition at 5 mM | Bifidobacterium longum | |
| Mg2+ | 65% inhibition at 5 mM | Bifidobacterium longum | |
| Mn2+ | 70% inhibition at 5 mM | Bifidobacterium longum | |
| additional information | no effect by 5 mM EDTA | Bifidobacterium longum | |
| Zn2+ | 20% inhibition at 5 mM | Bifidobacterium longum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic analysis, Km values for amylose and amylopectin of the recombinant His6-tagged enzyme are 9.2 mg/ml and 3.8 mg/ml, respectively, pH 5.0, 20°C | Bifidobacterium longum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slightly activating | Bifidobacterium longum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | A0A0M4N1K4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme 88.9fold from Escherichia coli strain MC1061 by nickel affinity chromatography | Bifidobacterium longum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.7 | - |
purified recombinant His6-tagged enzyme, pH 5.0, 20°C | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bifidobacterium longum | maltooligosaccharides | - |
? | |
| amylose + H2O | best substrate | Bifidobacterium longum | maltooligosaccharides | - |
? | |
| cycloamylose + H2O | - |
Bifidobacterium longum | maltooligosaccharides | - |
? | |
| glycogen + H2O | - |
Bifidobacterium longum | maltooligosaccharides | - |
? | |
| maltoheptaose + H2O | - |
Bifidobacterium longum | maltooligosaccharides | - |
? | |
| additional information | substrate specificity, overview. Reaction product analysis by anion exchange chromatography. The enzyme reaction products are in the range of glucose to maltotetraose, whereas maltooligosaccharides smaller than maltotetraose are partially hydrolyzed. Maltose is not degraded. Maltooligosaccharides larger than maltotetraose, such as maltoheptaose, cycloamylose, and amylose, are fully hydrolyzed. Relatively small cyclodextrins, such as alpha-cyclodextrin and beta-cyclodextrin, are not hydrolyzed by enzyme BiLA, although gamma-cyclodextrin is partially hydrolyzed. Macromolecules containing alpha-1,6 linkages, such as amylopectin, starch, and glycogen, are also partially hydrolyzed to maltooligosaccharides smaller than G4. Pullulan, a polysaccharide polymer consisting of maltotriose units, is not hydrolyzed | Bifidobacterium longum | ? | - |
? | |
| raw starch + H2O | from potato, corn, rice, and wheat | Bifidobacterium longum | maltooligosaccharides | - |
? | |
| soluble starch + H2O | 0.5% soluble starch | Bifidobacterium longum | maltooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, recombinant His6-tagged enzyme, SDS-PAGE | Bifidobacterium longum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BBG7_0117 | - |
Bifidobacterium longum |
| BiLA | - |
Bifidobacterium longum |
| bllj_0710 | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Bifidobacterium longum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | 80% of maximal activity at 10°C, 60% at 30°C, 40% at 40°C, and 20% at 50°C, inactivation at 60°C | Bifidobacterium longum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
amylopectin | recombinant His6-tagged enzyme, pH 5.0, 20°C | Bifidobacterium longum | |
| 116.1 | - |
amylose | recombinant His6-tagged enzyme, pH 5.0, 20°C | Bifidobacterium longum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
broad optimum at pH 4.5-6.0 | Bifidobacterium longum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8.5 | activity range, profile overview | Bifidobacterium longum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 13, GH13 | Bifidobacterium longum |
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