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Literature summary for 3.2.1.1 extracted from

  • Bhanja Dey, T.; Banerjee, R.
    Purification, biochemical characterization and application of alpha-amylase produced by Aspergillus oryzae IFO-30103 (2015), Biocatal. Agricult. Biotechnol., 4, 83-90 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
tert-butyl alcohol about 6% activating at 10-30% Aspergillus oryzae
Triton X-100 18.2% activating at 0.1% w/v Aspergillus oryzae

Application

Application Comment Organism
additional information the purified alpha-amylase is successfully utilized for the improvement of antioxidant potential of wheat Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10.7% inhibition at 10 mM Aspergillus oryzae
Ca2+ 16% activation at 1 mM, although Ca2+ is a good stabilizer it cannot activate the enzyme significantly, Ca2+ shows 41% inhibition of the enzyme at 10 mM Aspergillus oryzae
Cu2+ 29% inhibition at 1 mM Aspergillus oryzae
EDTA 30% inhibition at 10 mM, 20% at 5 mM Aspergillus oryzae
Fe3+ 9% inhibition at 1 mM Aspergillus oryzae
Hg2+ complete inhibition at 1 mM Aspergillus oryzae
additional information the enzyme activity is not affected by Tween 20, Tween 40, Tween 60, and Tween 80 at 0.1% w/v Aspergillus oryzae
SDS 61.8% inhibition at 0.1% w/v Aspergillus oryzae
Zn2+ 25% inhibition at 1 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, the purified alpha-amylase reveals Km and Vmax values of 0.5% and 1000 U/mg protein for soluble starch Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 16% activation at 1 mM, although Ca2+ is a good stabilizer it cannot activate the enzyme significantly, Ca2+ shows 41% inhibition of the enzyme at 10 mM Aspergillus oryzae
Co2+ 58% activation at 1 mM Aspergillus oryzae
Mg2+ 13% activation at 1 mM Aspergillus oryzae

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme is not or poorly affected by 10-30% of methanol, ethanol, and isoamylalcohol, and slightly activated by tert-butyl alcohol, overview Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae IFO-30103
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 7.1fold by acetone precipitation, gel filtration, ultrafiltration, and anion exchange chromatography Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
627.5
-
purified enzyme, pH 5.5, 50°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 55% activity compared to soluble starch Aspergillus oryzae ?
-
?
amylopectin + H2O 55% activity compared to soluble starch Aspergillus oryzae IFO-30103 ?
-
?
amylose + H2O 90% activity compared to soluble starch Aspergillus oryzae ?
-
?
amylose + H2O 90% activity compared to soluble starch Aspergillus oryzae IFO-30103 ?
-
?
corn starch + H2O 60% activity compared to soluble starch Aspergillus oryzae ?
-
?
glycogen + H2O 35% activity compared to soluble starch Aspergillus oryzae ?
-
?
glycogen + H2O 35% activity compared to soluble starch Aspergillus oryzae IFO-30103 ?
-
?
additional information no activity with pullulan and maltose Aspergillus oryzae ?
-
?
additional information no activity with pullulan and maltose Aspergillus oryzae IFO-30103 ?
-
?
potato starch + H2O 60% activity compared to soluble starch Aspergillus oryzae ?
-
?
rise starch + H2O 70% activity compared to soluble starch Aspergillus oryzae ?
-
?
soluble starch + H2O best substrate, 0.5% starch solution Aspergillus oryzae ?
-
?
soluble starch + H2O best substrate, 0.5% starch solution Aspergillus oryzae IFO-30103 ?
-
?
wheat starch + H2O 60% activity compared to soluble starch Aspergillus oryzae ?
-
?

Subunits

Subunits Comment Organism
? x * 51300, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 the purified enzyme exhibits 85.4% of maximal activity at 60°C, 45.7% at 70°C, and 22.3% activity at 80°C, profile overview Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 purified enzyme, 7 days, completely stable Aspergillus oryzae
50
-
purified enzyme, 10 mM Ca2+, 8 h 61.9% activity remaining. Without Ca2+, the enzyme loses 50% and 100% activity within 40 min and 120 min, respectively Aspergillus oryzae
60
-
purified enzyme, without Ca2+, 50% activity remaining after 10 min, inactivation after 30 min Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 activity range, profile overview Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7.2 purified enzyme, 4°C, 24 h, stable at Aspergillus oryzae