Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tert-butyl alcohol | about 6% activating at 10-30% | Aspergillus oryzae | |
Triton X-100 | 18.2% activating at 0.1% w/v | Aspergillus oryzae |
Application | Comment | Organism |
---|---|---|
additional information | the purified alpha-amylase is successfully utilized for the improvement of antioxidant potential of wheat | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10.7% inhibition at 10 mM | Aspergillus oryzae | |
Ca2+ | 16% activation at 1 mM, although Ca2+ is a good stabilizer it cannot activate the enzyme significantly, Ca2+ shows 41% inhibition of the enzyme at 10 mM | Aspergillus oryzae | |
Cu2+ | 29% inhibition at 1 mM | Aspergillus oryzae | |
EDTA | 30% inhibition at 10 mM, 20% at 5 mM | Aspergillus oryzae | |
Fe3+ | 9% inhibition at 1 mM | Aspergillus oryzae | |
Hg2+ | complete inhibition at 1 mM | Aspergillus oryzae | |
additional information | the enzyme activity is not affected by Tween 20, Tween 40, Tween 60, and Tween 80 at 0.1% w/v | Aspergillus oryzae | |
SDS | 61.8% inhibition at 0.1% w/v | Aspergillus oryzae | |
Zn2+ | 25% inhibition at 1 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, the purified alpha-amylase reveals Km and Vmax values of 0.5% and 1000 U/mg protein for soluble starch | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus oryzae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 16% activation at 1 mM, although Ca2+ is a good stabilizer it cannot activate the enzyme significantly, Ca2+ shows 41% inhibition of the enzyme at 10 mM | Aspergillus oryzae | |
Co2+ | 58% activation at 1 mM | Aspergillus oryzae | |
Mg2+ | 13% activation at 1 mM | Aspergillus oryzae |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the enzyme is not or poorly affected by 10-30% of methanol, ethanol, and isoamylalcohol, and slightly activated by tert-butyl alcohol, overview | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae IFO-30103 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 7.1fold by acetone precipitation, gel filtration, ultrafiltration, and anion exchange chromatography | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus oryzae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
627.5 | - |
purified enzyme, pH 5.5, 50°C | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 55% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
amylopectin + H2O | 55% activity compared to soluble starch | Aspergillus oryzae IFO-30103 | ? | - |
? | |
amylose + H2O | 90% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
amylose + H2O | 90% activity compared to soluble starch | Aspergillus oryzae IFO-30103 | ? | - |
? | |
corn starch + H2O | 60% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
glycogen + H2O | 35% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
glycogen + H2O | 35% activity compared to soluble starch | Aspergillus oryzae IFO-30103 | ? | - |
? | |
additional information | no activity with pullulan and maltose | Aspergillus oryzae | ? | - |
? | |
additional information | no activity with pullulan and maltose | Aspergillus oryzae IFO-30103 | ? | - |
? | |
potato starch + H2O | 60% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
rise starch + H2O | 70% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? | |
soluble starch + H2O | best substrate, 0.5% starch solution | Aspergillus oryzae | ? | - |
? | |
soluble starch + H2O | best substrate, 0.5% starch solution | Aspergillus oryzae IFO-30103 | ? | - |
? | |
wheat starch + H2O | 60% activity compared to soluble starch | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51300, SDS-PAGE | Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the purified enzyme exhibits 85.4% of maximal activity at 60°C, 45.7% at 70°C, and 22.3% activity at 80°C, profile overview | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 30 | purified enzyme, 7 days, completely stable | Aspergillus oryzae |
50 | - |
purified enzyme, 10 mM Ca2+, 8 h 61.9% activity remaining. Without Ca2+, the enzyme loses 50% and 100% activity within 40 min and 120 min, respectively | Aspergillus oryzae |
60 | - |
purified enzyme, without Ca2+, 50% activity remaining after 10 min, inactivation after 30 min | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | activity range, profile overview | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.2 | purified enzyme, 4°C, 24 h, stable at | Aspergillus oryzae |