Literature summary for 3.2.1.1 extracted from

Hameed, U.; Price, I.; Ikram-Ul-Ha, I.; Ke, A.; Wilson, D.; Mirza, O.
Functional characterization and crystal structure of thermostable amylase from Thermotoga petrophila, reveals high thermostability and an unusual form of dimerization (2017), Biochim. Biophys. Acta, 1865, 1237-1245 .

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli	Thermotoga petrophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Thermotoga petrophila

Inhibitors

Inhibitors	Comment	Organism	Structure
acarbose	activity is reduced to 56.4% in the presence of 0.00002 mM of inhibitor and inhibition reaches 77% at 0.00008 mM concentration	Thermotoga petrophila
Cu2+	1 mM, 99% loss of activity	Thermotoga petrophila
Fe2+	1 mM, 98% loss of activity	Thermotoga petrophila
Fe3+	1 mM, complete loss of activity	Thermotoga petrophila
Mg2+	1 mM, 14% loss of activity	Thermotoga petrophila
Mn2+	1 mM, complete loss of activity	Thermotoga petrophila
additional information	Li+ and Na+ do not show significant inhibitory effect	Thermotoga petrophila
Ni2+	1 mM, 99% loss of activity	Thermotoga petrophila
Pb2+	1 mM, complete loss of activity	Thermotoga petrophila

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	1 mM, increases activity by 20%	Thermotoga petrophila
K+	1 mM, increases activity by 16%	Thermotoga petrophila
Rb+	1 mM, increases activity by 10%	Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62720	-	electrospray ionisation	Thermotoga petrophila

Organism

Organism	UniProt	Comment	Textmining
Thermotoga petrophila	A5ILB0	-	-
Thermotoga petrophila DSM 13995	A5ILB0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga petrophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
starch + H2O	-	Thermotoga petrophila	dextrin	-	?
starch + H2O	-	Thermotoga petrophila DSM 13995	dextrin	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Thermotoga petrophila

Synonyms

Synonyms	Comment	Organism
Tp-AmyS	-	Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
98	-	-	Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
80	105	80°C: about 60% of maximal activity, 105°C: about 80% of maximal activity	Thermotoga petrophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Thermotoga petrophila

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Release 2023.1 (January 2023)