Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Oryzias latipes |
Crystallization (Comment) | Organism |
---|---|
His-tagged recombinant protein, to 2.85 A resolution, space group P3221, one protein molecule per asymmetric unit. One bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules. The overall structure is the TIM barrel structure with two additional domains, domain B protruding out of the barrel in the place of the beta3-alpha3 loop and domain C succeeding the catalytic barrel | Oryzias latipes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: NaCl up to 250 mM | Oryzias latipes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in the crystal structure, one bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules | Oryzias latipes | |
chloride | presence of chloride ions is essential for activity. 20 mM NaCl is sufficient for maximum activity. NaCl is not inhibitory below 250 mM | Oryzias latipes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryzias latipes | H2N0D4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | corn starch is preferred over wheat starch and sweet-potato starch. Potato starch is a poor substrate | Oryzias latipes | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Oryzias latipes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
melting temperature | Oryzias latipes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Oryzias latipes |