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Literature summary for 3.2.1.1 extracted from

  • Fukushima, T.; Mizuki, T.; Echigo, A.; Inoue, A.; Usami, R.
    Organic solvent tolerance of halophilic alpha-amylase from a Haloarchaeon, Haloarcula sp. strain S-1 (2005), Extremophiles, 9, 85-89.
    View publication on PubMed
Search for more literature for 3.2.1.1

General Stability

General Stability Organism
the enzyme is stable under NaCl concentrations between 1.8 and 4.8 M in the absence or presence of chloroform Haloarcula sp.

Inhibitors

Inhibitors Comment Organism Structure
acetone
-
 Haloarcula sp.
Cu2+ 20 mM Haloarcula sp.
Cyclohexane inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane Haloarcula sp.
cyclooctane inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane Haloarcula sp.
EDTA 5–15 mM, with or without chloroform Haloarcula sp.
ethanol
-
 Haloarcula sp.
Fe2+ 20 mM Haloarcula sp.
Fe3+ 20 mM Haloarcula sp.
additional information not inhibited by aromatic hydrocarbon (benzene, etc.) Haloarcula sp.
Ni2+ 20 mM Haloarcula sp.
Rb+ 20 mM Haloarcula sp.
Zn2+ 20 mM Haloarcula sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Haloarcula sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl optimal NaCl concentration in the absence of chloroform is 4.3 M. No activity is detected below 1.7 M NaCl. In the presence of chloroform, optimal NaCl concentration is 4.3 M, and activity is not detected below 0.9 M Haloarcula sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Haloarcula sp.
70000
-
 1 * 70000, SDS-PAGE Haloarcula sp.

Organic Solvent Stability

Organic Solvent Comment Organism
benzene stable in presence of Haloarcula sp.
chloroform stable in presence of Haloarcula sp.
n-decane stable in presence of Haloarcula sp.
n-nonane stable in presence of Haloarcula sp.
octane stable in presence of Haloarcula sp.
styrene stable in presence of Haloarcula sp.
toluene stable in presence of Haloarcula sp.
xylene stable in presence of Haloarcula sp.

Organism

Organism UniProt Comment Textmining
Haloarcula sp.
-
-
-
Haloarcula sp. S-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloarcula sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.07 mg of starch/min*mg Haloarcula sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Haloarcula sp. maltose + maltotriose + D-glucose major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose ?
starch + H2O
-
 Haloarcula sp. S-1 maltose + maltotriose + D-glucose major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Haloarcula sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in buffer containing 4.3 M NaCl, pH 7.0 Haloarcula sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
 pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, no loss of activity Haloarcula sp.
70
-
 pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, 50% loss of activity Haloarcula sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Haloarcula sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 25°C, 30 min, stable Haloarcula sp.