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Literature summary for 3.2.1.1 extracted from
- Gene analysis, expression, and characterization of an intracellular alpha-amylase from the extremely halophilic archaeon Haloarcula japonica (2013), Biosci. Biotechnol. Biochem., 77, 281-288.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Haloarcula japonica | Haloarcula japonica |
General Stability
| General Stability | Organism |
|---|---|
| halophilic enzyme, partially denatured at low salt concentrations, stable over a broad range of NaCl concentrations, from 0.5 to 4.5 M. Stability with KCl is equal to that with NaCl, indicating that the type of monovalent cation is not critical to the stability | Haloarcula japonica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Haloarcula japonica | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activity of the enzyme in KCl is equal to that in NACl, indicating that the type of monovalent cation is not critical to the activity | Haloarcula japonica |  |
| NaCl | maximal activity at 2.6 M NaCl. 83% and 95% of the maximum activity are observed at 0.6 and 4.2 M NaCl, respectively | Haloarcula japonica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72026 | - |
x * 72026, calculated from sequence | Haloarcula japonica |
| 83000 | - |
x * 83000, C-terminally 6*His-tagged recombinant enzyme | Haloarcula japonica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloarcula japonica | L8B068 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloarcula japonica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 68% of the activity with amylose | Haloarcula japonica | ? | - |
? | |
| amylose + H2O | - |
Haloarcula japonica | ? | - |
? | |
| glycogen + H2O | 22% of the activity with amylose | Haloarcula japonica | ? | - |
? | |
| soluble starch + H2O | 79% of the activity with amylose | Haloarcula japonica | ? | at the initial stage of the reaction, maltose and maltotriose are formed, then the amount of maltose increases with time | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72026, calculated from sequence | Haloarcula japonica |
| ? | x * 83000, C-terminally 6*His-tagged recombinant enzyme | Haloarcula japonica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MalA | - |
Haloarcula japonica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Haloarcula japonica |
| 45 | - |
at pH 6.5 | Haloarcula japonica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | 25°C: about 65% of maximal activity, 60°C: about 55% of maximal activity | Haloarcula japonica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to | Haloarcula japonica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Haloarcula japonica |
| 7.5 | - |
assay at | Haloarcula japonica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | pH 6.0: about 85% of maximal activity, pH 7.5: about 70% of maximal activity | Haloarcula japonica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 9.2 | stable | Haloarcula japonica |
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