Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in Saccharomyces cerevisiae strain AH22, subcloning in Escherichia coli strain TG1 | Saccharomycopsis fibuligera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saccharomycopsis fibuligera | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, recombinant enzyme, SDS-PAGE | Saccharomycopsis fibuligera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomycopsis fibuligera | D4P4Y7 | - |
- |
Saccharomycopsis fibuligera KZ | D4P4Y7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme contains about 2.5% of carbohydrate | Saccharomycopsis fibuligera |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Saccharomyces cerevisiae to homogeneity | Saccharomycopsis fibuligera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
amylose + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
amylose + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
beta-limit dextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
corn starch + H2O | boiled starch | Saccharomycopsis fibuligera | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
maltoheptaose + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
maltoheptaose + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
additional information | substrate specificity, overview | Saccharomycopsis fibuligera | ? | - |
? | |
additional information | substrate specificity, overview | Saccharomycopsis fibuligera KZ | ? | - |
? | |
pullulan + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
pullulan + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
raw starch + H2O | the recombinant enzyme shows low degradation of raw starch in a concentration-dependent manner | Saccharomycopsis fibuligera | ? | - |
? | |
rice starch + H2O | boiled starch | Saccharomycopsis fibuligera | ? | - |
? | |
soluble starch + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant enzyme, SDS-PAGE | Saccharomycopsis fibuligera |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycoside hydrolase family 13, subfamily 1 | Saccharomycopsis fibuligera |
Sfamy | - |
Saccharomycopsis fibuligera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Saccharomycopsis fibuligera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | activity range | Saccharomycopsis fibuligera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Saccharomycopsis fibuligera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.9 | 7 | activity range | Saccharomycopsis fibuligera |