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Literature summary for 3.2.1.1 extracted from
- Role of Val289 residue in the alpha-amylase of Bacillus amyloliquefaciens MTCC 610: An analysis by site directed mutagenesis (2010), J. Microbiol. Biotechnol., 20, 563-568.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1 Blue cells | Bacillus amyloliquefaciens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V289D | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
| V289E | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
| V289F | the mutant shows 48.9% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
| V289G | the mutant shows 14.5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
| V289I | the mutant shows 20% more hydrolytic activity than the wild type enzyme | Bacillus amyloliquefaciens |
| V289L | the mutant shows 36.4% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
| V289P | the mutant shows 2.2% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
| V289R | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
| V289S | the mutant shows 5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
| V289Y | the mutant shows 9.2% activity compared to the wild type enzyme, the mutant has acquired a transglycosylation activity, which results in the change of product profile of the reaction, giving a longer oligosaccharide | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus amyloliquefaciens MTCC 610 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltotetraose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? |  |
| maltotetraose + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | ? | - |
? | |
| starch + H2O | - |
Bacillus amyloliquefaciens | malto-oligosaccharides | - |
? | |
| starch + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | malto-oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AmyA | - |
Bacillus amyloliquefaciens |
| endo-1,4-alpha-D-glucan glucohydrolase | - |
Bacillus amyloliquefaciens |
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