Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus sp. |
General Stability | Organism |
---|---|
Ca2+ strongly stabilizes alpha-amylase while EDTA reduces its stability | Geobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 80% residual activity at 10 mM | Geobacillus sp. | |
Co2+ | 70% residual activity at 10 mM | Geobacillus sp. | |
Cu2+ | 86% residual activity at 10 mM | Geobacillus sp. | |
EDTA | 21% residual activity at 10 mM, alpha-amylase is completely inactivated after 6 min of incubation at 80°C in the presence of 5 mM EDTA | Geobacillus sp. | |
Fe3+ | 10% residual activity at 10 mM | Geobacillus sp. | |
Mg2+ | 40% residual activity at 10 mM | Geobacillus sp. | |
additional information | not inhibited by phytic acid | Geobacillus sp. | |
Ni2+ | 60% residual activity at 10 mM | Geobacillus sp. | |
Zn2+ | 64% residual activity at 10 mM | Geobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km value for starch is 3 mg/ml at 65°C using potato starch (1% (w/v)) as a substrate in 20 mM Tris-HCl, pH 7.4 | Geobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 120% relative activity at 10 mM | Geobacillus sp. | |
Ca2+ | 200% relative activity at 10 mM | Geobacillus sp. | |
Cr3+ | 140% relative activity at 10 mM | Geobacillus sp. | |
K+ | 105% relative activity at 10 mM | Geobacillus sp. | |
Mn2+ | 140% relative activity at 10 mM | Geobacillus sp. | |
additional information | not stimulated by Na+ | Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | - |
- |
- |
Geobacillus sp. LH8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono Q-Sepharose column chromatography | Geobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.6 | - |
after 13.6fold purification, at 65°C | Geobacillus sp. |
59 | - |
crude extract, at 65°C | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
potato starch + H2O | - |
Geobacillus sp. | malto-oligosaccharides | - |
? | |
potato starch + H2O | - |
Geobacillus sp. LH8 | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-alpha-D-glucan glucanohydrolase | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
in the absence of Ca2+ | Geobacillus sp. |
80 | - |
in the presence of 10 mM Ca2+ | Geobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | 29% activity is seen after 10 min at 85 and 90°C, respectively | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Geobacillus sp. |