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Literature summary for 3.2.1.1 extracted from
- The influence of N-glycosylation on biochemical properties of Amy1, an alpha-amylase from the yeast Cryptococcus flavus (2009), Carbohydr. Res., 344, 1682-1686.
General Stability
| General Stability | Organism |
|---|---|
| the non-glycosylated enzyme form is more sensitive to inactivation by both the proteolytic enzyme trypsin and high temperature | Saitozyma flava |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Saitozyma flava |  |
| Hg2+ | - |
Saitozyma flava | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the KM values of native and non-glycosylated alpha-amylases are 0.052 and 0.098 mg/ml | Saitozyma flava |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64500 | - |
deglycosylated enzyme, SDS-PAGE | Saitozyma flava |
| 68000 | - |
native enzyme, SDS-PAGE | Saitozyma flava |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Saitozyma flava | - |
malto-oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saitozyma flava | - |
isolate I-11 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylation is involved in the folding of Amy1 | Saitozyma flava |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography and Sephacryl S-100 gel filtration | Saitozyma flava |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Saitozyma flava | malto-oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMY1 | - |
Saitozyma flava |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saitozyma flava | Amy1 activity increases by 55% in the intracellular fraction after Cryptococcus flavus growth in the presence of 0.0005 mg/ml Cryptococcus tunicamycin | up |
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