Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana tabacum NT1 cells and in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
recombinant enzyme AmyN26, SDS-PAGE | Thermotoga maritima |
62060 | - |
calculated from amino acid sequence | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Thermotoga maritima | - |
malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
strain DSM 3109 | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Thermotoga maritima | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmyA | - |
Thermotoga maritima |
AmyN26 | recombinant enzyme | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
recombinant enzyme made from Escherichia coli | Thermotoga maritima |
90 | - |
recombinant enzyme made from Nicotiana tabacum | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 100 | there is no detectable activity of plant-made AmyN26 below 37°C when compared to the wild type. The plant-made enzyme retains 85% of its initial activity after 3 h incubation at 100°C, the recombinant enzyme is completely inactivated after 30 min under the same conditions | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermotoga maritima |