Any feedback?
Literature summary for 3.2.1.1 extracted from
- Biochemical properties of alpha-amylase from peel of Citrus sinensis cv. Abosora (2010), Appl. Biochem. Biotechnol., 160, 2054-2065.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 80% relative activity at 10 mM Ca2+ | Citrus sinensis |  |
| Cu2+ | 7% residual activity at 2 mM | Citrus sinensis | |
| Hg2+ | complete inhibition at 2 mM | Citrus sinensis | |
| Mg2+ | 82% residual activity at 2 mM | Citrus sinensis | |
| Mn2+ | 12% residual activity at 2 mM | Citrus sinensis | |
| Ni2+ | 73% residual activity at 2 mM | Citrus sinensis | |
| Zn2+ | 5% residual activity at 2 mM | Citrus sinensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 35% increase of activity at 2 mM | Citrus sinensis | |
| Ca2+ | the activity of the alpha-amylase AI is increased 1.5fold in the presence of 4 mM Ca2+ | Citrus sinensis | |
| additional information | Li+ has negligible effect on activity | Citrus sinensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration and SDS-PAGE | Citrus sinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Citrus sinensis | 73% activity compared to corn starch | malto-oligosaccharides | - |
? | |
| corn starch + H2O | Citrus sinensis | 100% activity | malto-oligosaccharides | - |
? | |
| dextrin + H2O | Citrus sinensis | 67% activity compared to corn starch | malto-oligosaccharides | - |
? | |
| glycogen + H2O | Citrus sinensis | 81% activity compared to corn starch | malto-oligosaccharides | - |
? | |
| additional information | Citrus sinensis | does not hydrolyze beta-cyclodextrin and dextran | ? | - |
? | |
| potato starch + H2O | Citrus sinensis | potato starch has low affinity toward alpha-amylase AI (12% activity compared to corn starch) | malto-oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus sinensis | - |
cultivar Abosora | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TEAE-cellulose column chromatography, carboxymethyl-cellulose column chromatography, and Sephacryl S-200 gel filtration | Citrus sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| peel | - |
Citrus sinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.956 | - |
crude extract, at 40°C | Citrus sinensis |
| 7.986 | - |
after 8.4fold purification, at 40°C | Citrus sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 73% activity compared to corn starch | Citrus sinensis | malto-oligosaccharides | - |
? | |
| corn starch + H2O | 100% activity | Citrus sinensis | malto-oligosaccharides | - |
? | |
| dextrin + H2O | 67% activity compared to corn starch | Citrus sinensis | malto-oligosaccharides | - |
? | |
| glycogen + H2O | 81% activity compared to corn starch | Citrus sinensis | malto-oligosaccharides | - |
? | |
| additional information | does not hydrolyze beta-cyclodextrin and dextran | Citrus sinensis | ? | - |
? | |
| potato starch + H2O | potato starch has low affinity toward alpha-amylase AI (12% activity compared to corn starch) | Citrus sinensis | malto-oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Citrus sinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-amylase AI | the enzyme is an endoamylase | Citrus sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Citrus sinensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | alpha-amylase AI maintains about 50% activity over a temperature range from 10-50°C | Citrus sinensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | the enzyme is thermally stable up to 40°C and inactivated at 70°C, the enzyme has lost 80% of its activity at 60°C | Citrus sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
in sodium acetate | Citrus sinensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
