Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli K12TB1 | Haliotis discus discus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 2 mM, pH 6.5, 50°C, 50% relative activity | Haliotis discus discus | |
FeSO4 | 2 mM, pH 6.5, 50°C, 47% relative activity | Haliotis discus discus | |
MgSO4 | 2 mM, pH 6.5, 50°C, 75% relative activity | Haliotis discus discus | |
MnCl2 | 2 mM, pH 6.5, 50°C, 40% relative activity | Haliotis discus discus | |
ZnSO4 | 2 mM, pH 6.5, 50°C, 37% relative activity | Haliotis discus discus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2 mM, pH 6.5, 50°C, 115% relative activity | Haliotis discus discus | |
EDTA | 2 mM, pH 6.5, 50°C, 40% relative activity | Haliotis discus discus | |
KCl | 2 mM, pH 6.5, 50°C, 105% relative activity | Haliotis discus discus | |
NaCl | 2 mM, pH 6.5, 50°C, 100% relative activity | Haliotis discus discus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
SDS-PAGE, as fusion protein 965000 Da, combined with the maltose binding protein with 42500 Da | Haliotis discus discus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliotis discus discus | B6RB08 | active site residues Asp214, Glu250, and Asp315, as well as the residues making up the calcium (Asn118, Arg175, Asp184, and His218) and chloride (Arg212, Asn313, and Arg351) binding sites are fully conserved in HdAmyI; disk abalone | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Haliotis discus discus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alimentary canal | highest transcription levels in the hepatopancreas, followed by digestive tract tissue | Haliotis discus discus | - |
hepatopancreas | highest transcription levels in the hepatopancreas, followed by digestive tract tissue | Haliotis discus discus | - |
additional information | no transcription in the gill, mantle, and abductor muscle | Haliotis discus discus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
0.1% starch as substrate | Haliotis discus discus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | pH 6.5, 50°C | Haliotis discus discus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE, as fusion protein 965000 Da, combined with the maltose binding protein with 42500 Da | Haliotis discus discus |
Synonyms | Comment | Organism |
---|---|---|
HdAmyI | - |
Haliotis discus discus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Haliotis discus discus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: 40% relative activity, 70°C: 50% relative activity, from 30 to 60°C the activity retains up to more than 70% | Haliotis discus discus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
up to more than 60% relative activity, incubated for 30 min, is stable, even for 1 h | Haliotis discus discus |
40 | - |
up to more than 60% relative activity, incubated for 30 min, is stable, even for 1 h | Haliotis discus discus |
50 | - |
up to more than 60% relative activity, incubated for 30 min | Haliotis discus discus |
60 | 70 | thermal inactivation occurs when the protein is incubated at 60-70°C for 30 min | Haliotis discus discus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Haliotis discus discus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | pH 3.5: ca. 45% relative activity, pH 9.5: ca. 30% relative activity, high activity in the range from pH 5.5 to 8.5 | Haliotis discus discus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haliotis discus discus | estimated by sequence calculation | - |
8.3 |