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Literature summary for 3.2.1.1 extracted from

  • Nikapitiya, C.; Oh, C.; Whang, I.; Kim, C.G.; Lee, Y.H.; Kim, S.J.; Lee, J.
    Molecular characterization, gene expression analysis and biochemical properties of alpha-amylase from the disk abalone, Haliotis discus discus (2009), Comp. Biochem. Physiol. B, 152, 271-281.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli K12TB1 Haliotis discus discus

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 2 mM, pH 6.5, 50°C, 50% relative activity Haliotis discus discus
FeSO4 2 mM, pH 6.5, 50°C, 47% relative activity Haliotis discus discus
MgSO4 2 mM, pH 6.5, 50°C, 75% relative activity Haliotis discus discus
MnCl2 2 mM, pH 6.5, 50°C, 40% relative activity Haliotis discus discus
ZnSO4 2 mM, pH 6.5, 50°C, 37% relative activity Haliotis discus discus

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 2 mM, pH 6.5, 50°C, 115% relative activity Haliotis discus discus
EDTA 2 mM, pH 6.5, 50°C, 40% relative activity Haliotis discus discus
KCl 2 mM, pH 6.5, 50°C, 105% relative activity Haliotis discus discus
NaCl 2 mM, pH 6.5, 50°C, 100% relative activity Haliotis discus discus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
SDS-PAGE, as fusion protein 965000 Da, combined with the maltose binding protein with 42500 Da Haliotis discus discus

Organism

Organism UniProt Comment Textmining
Haliotis discus discus B6RB08 active site residues Asp214, Glu250, and Asp315, as well as the residues making up the calcium (Asn118, Arg175, Asp184, and His218) and chloride (Arg212, Asn313, and Arg351) binding sites are fully conserved in HdAmyI; disk abalone
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Haliotis discus discus

Source Tissue

Source Tissue Comment Organism Textmining
alimentary canal highest transcription levels in the hepatopancreas, followed by digestive tract tissue Haliotis discus discus
-
hepatopancreas highest transcription levels in the hepatopancreas, followed by digestive tract tissue Haliotis discus discus
-
additional information no transcription in the gill, mantle, and abductor muscle Haliotis discus discus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
0.1% starch as substrate Haliotis discus discus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O pH 6.5, 50°C Haliotis discus discus ?
-
?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE, as fusion protein 965000 Da, combined with the maltose binding protein with 42500 Da Haliotis discus discus

Synonyms

Synonyms Comment Organism
HdAmyI
-
Haliotis discus discus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Haliotis discus discus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: 40% relative activity, 70°C: 50% relative activity, from 30 to 60°C the activity retains up to more than 70% Haliotis discus discus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
up to more than 60% relative activity, incubated for 30 min, is stable, even for 1 h Haliotis discus discus
40
-
up to more than 60% relative activity, incubated for 30 min, is stable, even for 1 h Haliotis discus discus
50
-
up to more than 60% relative activity, incubated for 30 min Haliotis discus discus
60 70 thermal inactivation occurs when the protein is incubated at 60-70°C for 30 min Haliotis discus discus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Haliotis discus discus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9.5 pH 3.5: ca. 45% relative activity, pH 9.5: ca. 30% relative activity, high activity in the range from pH 5.5 to 8.5 Haliotis discus discus

pI Value

Organism Comment pI Value Maximum pI Value
Haliotis discus discus estimated by sequence calculation
-
8.3