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Literature summary for 3.2.1.1 extracted from

  • Valencia-Jimenez, A.; Arboleda, J.W.; Lopez Avila, A.; Grossi-de-Sa, M.F.
    Digestive alpha-amylases from Tecia solanivora larvae (Lepidoptera: Gelechiidae): response to pH, temperature and plant amylase inhibitors (2008), Bull. Entomol. Res., 98, 575-579.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
inhibitor from Amaranthus hybrid no effect on alpha-amlyase at pH 6.0, but high inhibitory effect with maximal 80% at pH 9.0, 50°C Tecia solanivora
inhibitor from Phaseolus coccineus 35619 no effect at pH 9.0, inhibits the enzyme activity up to 78% at pH 6.0, 50°C Tecia solanivora
inhibitor from Phaseolus vulgaris cv. Radical no effect at pH 9.0, inhibits the enzyme activity up to 87% at pH 6.0, 50°C Tecia solanivora

Organism

Organism UniProt Comment Textmining
Tecia solanivora
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Tecia solanivora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O 50°C, pH 6.0 or 9.0 Tecia solanivora ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Tecia solanivora

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: ca. 80% relative activity, 70°C: ca. 20% relative activity Tecia solanivora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at pH 6.0 and 9.0 Tecia solanivora
9
-
optimal range between pH 7 and 10 with a peak at pH 9, assay at pH 6.0 and 9.0 Tecia solanivora

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5: ca. 50% relative activity, pH 11: below ca. 60% relative activity Tecia solanivora

pI Value

Organism Comment pI Value Maximum pI Value
Tecia solanivora isoeletric focusing, three major alpha-amylases with pI-values of 5.3, 5.7, and 5.98
-
5.3
Tecia solanivora isoeletric focusing, three major alpha-amylases with pI-values of 5.3, 5.7, and 5.98
-
5.7
Tecia solanivora isoeletric focusing, three major alpha-amylases with pI-values of 5.3, 5.7, and 5.98
-
5.98