Literature summary for 3.2.1.1 extracted from

Gyemant, G.; Zajacz, A.; Becsi, B.; Ragunath, C.; Ramasubbu, N.; Erdodi, F.; Batta, G.; Kandra, L.
Evidence for pentagalloyl glucose binding to human salivary alpha-amylase through aromatic amino acid residues (2009), Biochim. Biophys. Acta, 1794, 291-296.

Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression and purification carried out using a Bac-To-Bac Baculovirus Expression System	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
W58L	reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM pentagalloyl glucose inhibitor concentration, respectively, pH 6.0, 37°C	Homo sapiens
Y151M	reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM pentagalloyl glucose inhibitor concentration, respectively, pH 6.0, 37°C	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,2,3,4,6-pentagalloyl-beta-D-glucose	mixed non-competitive inhibition, KEI: 0.0026 mM, KEIS: 0.0039 mM, tested in a concentration range of 0.04 to 0.5 mM, reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM inhibitor concentration, respectively, pH 6.0, 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, expression and purification carried out using a Bac-To-Bac Baculovirus Expression System	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
saliva	type IXA	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-chloro-4-nitrophenyl alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranoside + H2O	-	Homo sapiens	2-chloro-4-nitrophenol + alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranose	the increase of absorbance of 2-chloro-4-nitrophenol liberated by HSA is measured continuously at 400 nm	?

Synonyms

Synonyms	Comment	Organism
HAS	human salivary alpha-amylase	Homo sapiens
human salivary alpha-amylase	commercial preparation	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
6	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00235	-	1,2,3,4,6-pentagalloyl-beta-D-glucose	pH 6.0, 37°C, the mutants Y151M and W58L exhibit reduced inhibitory efficiency with 92 and 97% remaining enzyme activity at 0.00235 mM inhibitor concentration, respectively, pH 6.0, 37°C	Homo sapiens

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Release 2023.1 (January 2023)