KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
soluble starch | Km: 45 mg/ml, Vmax 9 mg/ml/min | Thermococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | isolated from a deep-sea hydrothermal vent | Thermococcus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Ca2+ | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51400 | - |
SDS-PAGE | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
extremely thermophilic anaerobic archaeon | - |
Thermococcus sp. HJ21 | - |
extremely thermophilic anaerobic archaeon | - |
Purification (Comment) | Organism |
---|---|
3fold with a yield of 9% | Thermococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | isolated from a deep-sea hydrothermal vent | Thermococcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
8.3 U/mg purified enzyme, pH 5.0, 95°C | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | pH 5.0, 95°C | Thermococcus sp. | ? | varying amounts of maltooligosaccharides, depending on reaction time | ? | |
soluble starch + H2O | pH 5.0, 95°C | Thermococcus sp. HJ21 | ? | varying amounts of maltooligosaccharides, depending on reaction time | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51400, SDS-PAGE | Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
more than 80% of the maximum activity remains at pH 4.5 and 95°C | Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable at pH 5.0-7.0 for 1 h | Thermococcus sp. |
90 | - |
half-life of the enzyme 5 h, at pH 5.0-7.0 stable for 1 h | Thermococcus sp. |
100 | - |
over 40% and 30% of the enzyme activity remains after incubation at 100°C for 2 and 3 h, respectively. At pH 5.0-6.0 stable for 1 h | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | less than 10% relative activity at pH 4, and around 10% at pH 9 | Thermococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
more than 80% of the maximum activity remains at pH 4.5 and 95°C | Thermococcus sp. |
5 | 6 | stable at 100°C for 1 h | Thermococcus sp. |
5 | 7 | stable at 80°C and 90°C for 1 h | Thermococcus sp. |