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Literature summary for 3.2.1.1 extracted from

  • Lee, S.; Mouri, Y.; Minoda, M.; Oneda, H.; Inouye, K.
    Comparison of the wild-type alpha-amylase and its variant enzymes in Bacillus amyloliquefaciens in activity and thermal stability, and insights into engineering the thermal stability of Bacillus alpha-amylase (2006), J. Biochem., 139, 1007-1015.
    View publication on PubMed
Search for more literature for 3.2.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 0.24 mg/ml for soluble starch, variant enzyme M77 Bacillus amyloliquefaciens
additional information
-
 additional information 0.34 mg/ml for soluble starch, wild-type enzyme Bacillus amyloliquefaciens
additional information
-
 additional information 0.63 mg/ml for soluble starch, variant enzyme 21B Bacillus amyloliquefaciens
additional information
-
 additional information 0.8 mg/ml for soluble starch, variant enzyme M111 Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 optimal temperature in absence of Ca2+, variant enzyme 21B Bacillus amyloliquefaciens
40
-
 optimal temperature in absence of Ca2+, variant enzyme M111 Bacillus amyloliquefaciens
50 60 optimal temperature in presence of 5 mM Ca2+, variant enzyme 21B Bacillus amyloliquefaciens
50 60 optimal temperature in presence of 5 mM Ca2+, variant enzyme M111 Bacillus amyloliquefaciens
60
-
 optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, variant enzyme M77 Bacillus amyloliquefaciens
60
-
 optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, wild-type enzyme Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information 503 per s for soluble starch, variant enzyme M77 Bacillus amyloliquefaciens
additional information
-
 additional information 586 per s for soluble starch, wild-type enzyme Bacillus amyloliquefaciens
additional information
-
 additional information 656 per s for soluble starch, variant enzyme M111 Bacillus amyloliquefaciens
additional information
-
 additional information 722 per s for soluble starch, variant enzyme 21B Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 wild-type enzyme Bacillus amyloliquefaciens
6
-
 variant enzyme 21B Bacillus amyloliquefaciens
6
-
 variant enzyme M111 Bacillus amyloliquefaciens
6
-
 variant enzyme M77 Bacillus amyloliquefaciens