Application | Comment | Organism |
---|---|---|
biotechnology | the thermotolerant, glucose tolerant maltooligosaccharide-forming alpha-amylase is potent for biotechnological application | Fusicoccum sp. |
Cloned (Comment) | Organism |
---|---|
- |
Fusicoccum sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 2 mM, complete inhibition | Fusicoccum sp. | |
glucose | 1 M, 34% inhibition. No inhibition up to 0.25 M | Fusicoccum sp. | |
maltose | 0.25 M, 69% inhibition | Fusicoccum sp. | |
Mn2+ | 2 mM, about 70% inhibition | Fusicoccum sp. | |
Ni2+ | 2 mM, about 15% inhibition | Fusicoccum sp. | |
Zn2+ | 2 mM, about 85% inhibition | Fusicoccum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value: 5.2 mg/ml for soluble starch, 14.46 mg/ml for beta-cyclodextrin, 9.18 mg/ml for gamma-cyclodextrin | Fusicoccum sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Fusicoccum sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, about 1.2fold activation | Fusicoccum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Fusicoccum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusicoccum sp. | - |
- |
- |
Fusicoccum sp. BCC4124 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusicoccum sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
838.9 | - |
- |
Fusicoccum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + H2O | - |
Fusicoccum sp. | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Fusicoccum sp. BCC4124 | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Fusicoccum sp. | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Fusicoccum sp. BCC4124 | ? | - |
? | |
maltoheptaose + H2O | - |
Fusicoccum sp. | D-glucose + maltose + maltotriose | - |
? | |
maltohexaose + H2O | - |
Fusicoccum sp. | D-glucose + maltose + maltotriose | - |
? | |
maltopentaose + H2O | - |
Fusicoccum sp. | glucose + maltose + maltotriose | - |
? | |
maltotetraose + H2O | - |
Fusicoccum sp. | glucose + maltose + maltotriose | - |
? | |
maltotriose + H2O | - |
Fusicoccum sp. | maltose + D-glucose | - |
? | |
maltotriose + H2O | - |
Fusicoccum sp. BCC4124 | maltose + D-glucose | - |
? | |
additional information | trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose | Fusicoccum sp. | ? | - |
? | |
additional information | trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose | Fusicoccum sp. BCC4124 | ? | - |
? | |
soluble starch + H2O | maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose | Fusicoccum sp. | ? | - |
? | |
soluble starch + H2O | maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose | Fusicoccum sp. BCC4124 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Fusicoccum sp. |
Synonyms | Comment | Organism |
---|---|---|
Amy-FC1 | - |
Fusicoccum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Fusicoccum sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | 30°C: about 60% of maximal activity, 75°C: about 85% of maximal activity | Fusicoccum sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, less than 5% loss of activity | Fusicoccum sp. |
60 | - |
1 h, complete inactivation in absence of substrate, less than 10% inactivation in presence of soluble starch. In presence of Ca2+ and Sr2+, Amy-FC1 retains full activity after 1 h and more than 80% activity after 5 h | Fusicoccum sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
soluble starch | - |
Fusicoccum sp. | |
283.3 | - |
beta-cyclodextrin | - |
Fusicoccum sp. | |
485 | - |
beta-cyclodextrin | - |
Fusicoccum sp. | |
561.7 | - |
soluble starch | - |
Fusicoccum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Fusicoccum sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
50°C, 1 h, about 40% loss of activity | Fusicoccum sp. |
7 | 8 | 50°C, 1 h, no loss of activity | Fusicoccum sp. |
9 | - |
50°C, 1 h, about 60% loss of activity | Fusicoccum sp. |