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Literature summary for 3.2.1.1 extracted from

  • Liu, Y.H.; Lu, F.P.; Li, Y.; Yin, X.B.; Wang, Y.; Gao, C.
    Characterisation of mutagenised acid-resistant alpha-amylase expressed in Bacillus subtilis WB600 (2008), Appl. Microbiol. Biotechnol., 78, 85-94.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
L134R/S320A the mutagenised protein is more acid resistant than the native protein. The optimum pH and stable range of pH with the mutagenised protein is 4.5 and 4.0 to 6.5, respectively, compared with pH 6.5 and 5.5 to 7.0 as the favorite pH and pH stability range of the native protein Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 23% inhibition of wild-type enzyme, 28% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Cd2+ 1 mM, 33% inhibition of wild-type enzyme, 48% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Cu2+ 1 mM, 55% inhibition of wild-type enzyme, 49% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Hg2+ 1 mM, complete inhibition of wild-type enzyme and mutant enzyme L134R/S320A Bacillus subtilis
Mg2+ 1 mM, 11% inhibition of wild-type enzyme, 5% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Mn2+ 1 mM, 20% inhibition of wild-type enzyme, 15% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Ni2+ 1 mM, 23% inhibition of wild-type enzyme, 24% inhibition of mutant enzyme L134R/S320A Bacillus subtilis
Zn2+ 1 mM, 28% inhibition of wild-type enzyme, 37% inhibition of mutant enzyme L134R/S320A Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.35fold activation of wild-type enzyme, 1.24fold activation of mutant enzyme L134R/S320A Bacillus subtilis
Cs+ 1 mM, 1.13fold activation of wild-type enzyme Bacillus subtilis
Sr2+ 1 mM, 1.11fold activation of wild-type enzyme, no activation of mutant enzyme L134R/S320A Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis WB600
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzyme L134R/S320A Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Bacillus subtilis dextrin
-
 ?
starch + H2O
-
 Bacillus subtilis WB600 dextrin
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 mutant enzyme L134R/S320A Bacillus subtilis
6.5
-
 wild-type enzyme Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 pH 3.5: about 50% of maximal activity, pH 7.0: about 70% of maximal activity, mutant enzyme L134R/S320A Bacillus subtilis
5 7 pH 5.0: about 70% of maximal activity, pH 7.0: about 90% of maximal activity, wild-type enzyme Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6.5 70°C, 1 h, stable pH-range for mutant enzyme L134R/S320A Bacillus subtilis
5.5 7 70°C, 1 h, stable pH-range for wild-type enzyme Bacillus subtilis