Literature summary for 3.2.1.1 extracted from

Grzybowska, B.; Szweda, P.; Synowiecki, J.
Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme (2004), Mol. Biotechnol., 26, 101-110.

Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21(DE3) as soluble intein fusion protein	Pyrococcus woesei

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus woesei	-	DSM 3773	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble intein fusion protein partially from Escherichia coli strain BL21(DE3) by heat treatment at 75°C, cleavage of intein fusion protein	Pyrococcus woesei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycogen + H2O	49.5% of the activity with starch	Pyrococcus woesei	maltose + ?	main product	?
additional information	no activity with pullulan	Pyrococcus woesei	?	-	?
starch + H2O	preferred substrate	Pyrococcus woesei	maltose + ?	main product	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermostable enzyme	Pyrococcus woesei
90	-	partially purified recombinant enzyme, half-life is 11 h, at pH 5.6 in 0.05 M acetate buffer	Pyrococcus woesei
110	-	partially purified recombinant enzyme, half-life is 3.5 h, at pH 5.6 in 0.05 M acetate buffer	Pyrococcus woesei
120	-	partially purified recombinant enzyme, 2 h, at pH 5.6 in 0.05 M acetate buffer, 24% remaining activity	Pyrococcus woesei

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Release 2023.1 (January 2023)