Cloned (Comment) | Organism |
---|---|
gene LSA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Lipomyces starkeyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Lipomyces starkeyi | |
Cu2+ | - |
Lipomyces starkeyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Lipomyces starkeyi | |
Mg2+ | stimulates | Lipomyces starkeyi | |
additional information | no effect by 1 mM EGTA | Lipomyces starkeyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68709 | - |
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE | Lipomyces starkeyi |
73000 | - |
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE | Lipomyces starkeyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lipomyces starkeyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin G5 + H2O | - |
Lipomyces starkeyi | maltodextrin G2 + maltodextrin G3 | - |
? | |
maltodextrin G6 + H2O | - |
Lipomyces starkeyi | maltodextrin G2 + maltodextrin G4 | - |
? | |
maltodextrin G6 + H2O | - |
Lipomyces starkeyi | 2 maltodextrin G3 | - |
? | |
maltodextrin G7 + H2O | - |
Lipomyces starkeyi | maltodextrin G3 + maltodextrin G4 | - |
? | |
additional information | no activity with maltodextrins of G2-G4 | Lipomyces starkeyi | ? | - |
? | |
starch + H2O | soluble starch | Lipomyces starkeyi | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE | Lipomyces starkeyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lipomyces starkeyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Lipomyces starkeyi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 16 h, purified recombinant His-tagged enzyme, 90% stable | Lipomyces starkeyi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
acarbose | pH 6.0, 40°C, recombinant His-tagged enzyme | Lipomyces starkeyi |