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Literature summary for 3.2.1.1 extracted from

  • Najafi, M.F.; Kembhavi, A.
    One step purification and characterization of an extracellular alpha-amylase from marine Vibrio sp. (2005), Enzyme Microb. Technol., 36, 535-539.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag2+
-
Vibrio sp.
Cu2+
-
Vibrio sp.
EDTA
-
Vibrio sp.
EGTA
-
Vibrio sp.
Hg2+
-
Vibrio sp.
Zn2+
-
Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Vibrio sp.
1.7
-
soluble starch pH 6.5, 60°C Vibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Vibrio sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Vibrio sp.
Co2+ activates Vibrio sp.
Fe2+ activates Vibrio sp.
Fe3+ activates slightly Vibrio sp.
Mg2+ activates Vibrio sp.
Mn2+ activates Vibrio sp.
Pb2+ activates slightly Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52480
-
x * 52480, SDS-PAGE Vibrio sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Vibrio sp. soluble starch malto-oligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
marine
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein determination of carbohydrate content of the enzyme Vibrio sp.

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 163.5fold from culture medium in a one-step process by starch affinity chromatography to homogeneity Vibrio sp.

Reaction

Reaction Comment Organism Reaction ID
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose mode of action Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
327
-
purified enzyme Vibrio sp.

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 20 mM MES, pH 6.5, at least 4 months without loss of activity Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Vibrio sp. ?
-
?
starch + H2O soluble starch Vibrio sp. malto-oligosaccharides
-
?

Subunits

Subunits Comment Organism
? x * 52480, SDS-PAGE Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Vibrio sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60
-
Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, purified enzyme, stable Vibrio sp.
70
-
30 min, loss of 80% activity Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Vibrio sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 50% of maximal activity at pH 4.5 and pH 8.5 Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 4°C, 24 h, purified enzyme, stable Vibrio sp.