Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | - |
Vibrio sp. | |
Cu2+ | - |
Vibrio sp. | |
EDTA | - |
Vibrio sp. | |
EGTA | - |
Vibrio sp. | |
Hg2+ | - |
Vibrio sp. | |
Zn2+ | - |
Vibrio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Vibrio sp. | |
1.7 | - |
soluble starch | pH 6.5, 60°C | Vibrio sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Vibrio sp. | |
Co2+ | activates | Vibrio sp. | |
Fe2+ | activates | Vibrio sp. | |
Fe3+ | activates slightly | Vibrio sp. | |
Mg2+ | activates | Vibrio sp. | |
Mn2+ | activates | Vibrio sp. | |
Pb2+ | activates slightly | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52480 | - |
x * 52480, SDS-PAGE | Vibrio sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Vibrio sp. | soluble starch | malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
marine | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | determination of carbohydrate content of the enzyme | Vibrio sp. |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 163.5fold from culture medium in a one-step process by starch affinity chromatography to homogeneity | Vibrio sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | mode of action | Vibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
327 | - |
purified enzyme | Vibrio sp. |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 20 mM MES, pH 6.5, at least 4 months without loss of activity | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Vibrio sp. | ? | - |
? | |
starch + H2O | soluble starch | Vibrio sp. | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52480, SDS-PAGE | Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Vibrio sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | - |
Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, purified enzyme, stable | Vibrio sp. |
70 | - |
30 min, loss of 80% activity | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Vibrio sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | 50% of maximal activity at pH 4.5 and pH 8.5 | Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 4°C, 24 h, purified enzyme, stable | Vibrio sp. |