Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, activity profile | Mytilus galloprovincialis | |
additional information | the enzyme is not affected by Mn2+ at 1-100 mM | Mytilus galloprovincialis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme forms I and II, gel filtration, overview | Mytilus galloprovincialis |
66000 | - |
x * 66000, enzyme form II, SDS-PAGE | Mytilus galloprovincialis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mytilus galloprovincialis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from mantle tissue by insoluble starch affinity chromatography and gel filtration, resolution of two enzyme forms, an aggregated form I and a monomeric form II, 142.4fold and 26.5fold purification, respectively | Mytilus galloprovincialis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mantle | - |
Mytilus galloprovincialis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
purified enzyme form II | Mytilus galloprovincialis |
42 | - |
purified enzyme form I | Mytilus galloprovincialis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Mytilus galloprovincialis | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 66000, enzyme form II, SDS-PAGE | Mytilus galloprovincialis |
More | the enzyme occurs in two forms, an aggregated form I and a monomeric form II, the latter is more abundant, but unstable | Mytilus galloprovincialis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile | Mytilus galloprovincialis |
35 | - |
enzyme form II | Mytilus galloprovincialis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Mytilus galloprovincialis |
6.5 | - |
enzyme form II | Mytilus galloprovincialis |