Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 98% inhibition of isozyme alpha-amylase I at 0.5 mM | Heterorhabditis bacteriophora | |
CoCl2 | 21% inhibition of isozyme alpha-amylase I at 0.5 mM, 29% at 5 mM | Heterorhabditis bacteriophora | |
EDTA | 92% inhibition of isozyme alpha-amylase I at 1 mM | Heterorhabditis bacteriophora | |
iodoacetic acid | 88% inhibition of isozyme alpha-amylase I at 0.5 mM | Heterorhabditis bacteriophora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
starch | pH 7.0, 40°C, alpha-amylase I | Heterorhabditis bacteriophora | |
9.6 | - |
glycogen | pH 7.0, 40 °C, alpha-amylase I | Heterorhabditis bacteriophora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 76% activation of isozyme alpha-amylase I at 1 mM | Heterorhabditis bacteriophora | |
additional information | alpha-amylase I is no affected by NaNO3, BaCl2, and MgCl2 | Heterorhabditis bacteriophora | |
NaCl | 18% activation of isozyme alpha-amylase I at 1 mM, 21% at 10 mM | Heterorhabditis bacteriophora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, alpha-amylase I, SDS-PAGE | Heterorhabditis bacteriophora |
47000 | - |
alpha-amylase I, gel filtration | Heterorhabditis bacteriophora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
amylose + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
glycogen + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterorhabditis bacteriophora | - |
entomopathogenic parasitic nematode, strain EGG, three isozymes | - |
Purification (Comment) | Organism |
---|---|
separation of three native isozymes from the infective juvenile of the nematode, purification of isozyme alpha-amylase I 74fold to homogeneity, by two steps of ion exchange chromatography and gel filtration | Heterorhabditis bacteriophora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | infective juvenile of the nematode, three isozymes, alpha-amylase I is the major isozyme | Heterorhabditis bacteriophora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
98.4 | - |
purified alpha-amylase I | Heterorhabditis bacteriophora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
amylose + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
amylose + H2O | preferred substrate | Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
glycogen + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
glycogen + H2O | substrate from oyster | Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, alpha-amylase I, SDS-PAGE | Heterorhabditis bacteriophora |
Synonyms | Comment | Organism |
---|---|---|
alpha-amylase I | - |
Heterorhabditis bacteriophora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isozyme alpha-amylase I | Heterorhabditis bacteriophora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified alpha-amylase I, stable up to, unstable above | Heterorhabditis bacteriophora |
50 | - |
30 min, loss of 62% activity of purified alpha-amylase I | Heterorhabditis bacteriophora |
60 | - |
30 min, loss of 78% activity of purified alpha-amylase I | Heterorhabditis bacteriophora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isozyme alpha-amylase I | Heterorhabditis bacteriophora |