Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slightly activates the recombinant enzyme | Thermotoga maritima | |
DTT | recombinant enzyme, activates 3fold at 5-10 mM | Thermotoga maritima | |
L-cysteine | slightly activates the recombinant enzyme | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
gene amyC, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 90% inhibition at 5 mM, reversible by addition of Ca2+ or Mg2+ | Thermotoga maritima | |
Ca2+ | added alone Ca2+ is inhibitory | Thermotoga maritima | |
EDTA | complete inhibition at 0.1 mM, reversible by addition of Ca2+ or Mg2+ | Thermotoga maritima | |
EGTA | complete inhibition at 0.1 mM, reversible by addition of Ca2+ or Mg2+ | Thermotoga maritima | |
Mg2+ | added alone Mg2+ is inhibitory | Thermotoga maritima | |
additional information | the enzyme is inhibited by several metal ions | Thermotoga maritima | |
NaCl | 50% inhibition at 50 mM | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermotoga maritima | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE | Thermotoga maritima |
62859 | - |
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE | Thermotoga maritima |
241000 | - |
recombinant AmyC, gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene amyC or tm1438 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
gene amyC or tm1438 | - |
Purification (Comment) | Organism |
---|---|
recombinant AmyC from Escherichia coli | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | 1.6 | purified | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | best substrate for AmyC, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? | |
amylose + H2O | best substrate for AmyC, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? | |
beta-limit-dextrin + H2O | 28% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? | |
beta-limit-dextrin + H2O | 28% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? | |
maltotriose + H2O | hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima | maltose + D-glucose | - |
? | |
maltotriose + H2O | hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | maltose + D-glucose | - |
? | |
additional information | hydrolysis of alpha-1,4-glucosidic linkages, glycogen and beta-cyclodextrin are poor substrates for AmyC, no activity with pullulan and maltose | Thermotoga maritima | ? | - |
? | |
additional information | hydrolysis of alpha-1,4-glucosidic linkages, glycogen and beta-cyclodextrin are poor substrates for AmyC, no activity with pullulan and maltose | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
starch + H2O | soluble starch, 68% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? | |
starch + H2O | soluble starch, 68% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | D-glucose + maltose + maltotriose + maltodextrins | small amount of longer maltodextrins, degradation process via malto-oligosaccharides | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
alkaline alpha-amylase | - |
Thermotoga maritima |
AmyC | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
6 h, 80% remaining activity | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thermotoga maritima |