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Literature summary for 3.2.1.1 extracted from

  • Ballschmiter, M.; Fuetterer, O.; Liebl, W.
    Identification and characterization of a novel intracellular alkaline alpha-amylase from the hyperthermophilic bacterium Thermotoga maritima MSB8 (2006), Appl. Environ. Microbiol., 72, 2206-2211.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slightly activates the recombinant enzyme Thermotoga maritima
DTT recombinant enzyme, activates 3fold at 5-10 mM Thermotoga maritima
L-cysteine slightly activates the recombinant enzyme Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
gene amyC, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
ATP 90% inhibition at 5 mM, reversible by addition of Ca2+ or Mg2+ Thermotoga maritima
Ca2+ added alone Ca2+ is inhibitory Thermotoga maritima
EDTA complete inhibition at 0.1 mM, reversible by addition of Ca2+ or Mg2+ Thermotoga maritima
EGTA complete inhibition at 0.1 mM, reversible by addition of Ca2+ or Mg2+ Thermotoga maritima
Mg2+ added alone Mg2+ is inhibitory Thermotoga maritima
additional information the enzyme is inhibited by several metal ions Thermotoga maritima
NaCl 50% inhibition at 50 mM Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Thermotoga maritima 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE Thermotoga maritima
62859
-
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE Thermotoga maritima
241000
-
recombinant AmyC, gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
gene amyC or tm1438
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
gene amyC or tm1438
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AmyC from Escherichia coli Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3 1.6 purified Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + H2O best substrate for AmyC, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?
amylose + H2O best substrate for AmyC, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?
beta-limit-dextrin + H2O 28% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?
beta-limit-dextrin + H2O 28% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?
maltotriose + H2O hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima maltose + D-glucose
-
?
maltotriose + H2O hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltose + D-glucose
-
?
additional information hydrolysis of alpha-1,4-glucosidic linkages, glycogen and beta-cyclodextrin are poor substrates for AmyC, no activity with pullulan and maltose Thermotoga maritima ?
-
?
additional information hydrolysis of alpha-1,4-glucosidic linkages, glycogen and beta-cyclodextrin are poor substrates for AmyC, no activity with pullulan and maltose Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
starch + H2O soluble starch, 68% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?
starch + H2O soluble starch, 68% of the activity with amylose, hydrolysis of alpha-1,4-glucosidic linkages Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-glucose + maltose + maltotriose + maltodextrins small amount of longer maltodextrins, degradation process via malto-oligosaccharides ?

Subunits

Subunits Comment Organism
tetramer 4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
alkaline alpha-amylase
-
Thermotoga maritima
AmyC
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
6 h, 80% remaining activity Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Thermotoga maritima