Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55331 | - |
x * 55331, deduced from nucleotide sequence, native ABA | Bacillus sp. (in: Bacteria) |
57000 | - |
x * 57000, SDS-PAGE, native ABA | Bacillus sp. (in: Bacteria) |
74000 | - |
x * 74000, SDS-PAGE, recombinant ABA, due to glycosylation by Pichia pastoris | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus sp. (in: Bacteria) | - |
fragments of starch | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ABA, diafiltration, Q-Sepharose | Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
4200 units/mg, one unit is defined as the amount of alpha-amylase that gives an increase of 0.001 in A620/ml for 15 min in a total volume of 5 ml | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Bacillus sp. (in: Bacteria) | fragments of starch | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55331, deduced from nucleotide sequence, native ABA | Bacillus sp. (in: Bacteria) |
? | x * 57000, SDS-PAGE, native ABA | Bacillus sp. (in: Bacteria) |
? | x * 74000, SDS-PAGE, recombinant ABA, due to glycosylation by Pichia pastoris | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
ABA | - |
Bacillus sp. (in: Bacteria) |
alkalophilic Bacillus alpha-amylase | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
recombinant ABA, 50-60% loss of activity after 1 h, complete loss of activity after 100 min, native ABA, 20% loss of activity after 1 h, approx. 50% loss of activity after 100 min | Bacillus sp. (in: Bacteria) |
85 | - |
complete loss of activity after 5 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant ABA, at 37°C | Bacillus sp. (in: Bacteria) |
7 | - |
deglycosylated recombinant ABA, at 37°C | Bacillus sp. (in: Bacteria) |
8 | - |
native ABA, at 37°C | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | native ABA at 37°C, approx. 30% of maximal activity at pH 5.0, approx. 90% of maximal activity at pH 10.0 | Bacillus sp. (in: Bacteria) |
5 | 10 | recombinant ABA at 37°C, approx. 30% of maximal activity at pH 5.0, approx. 30% of maximal activity at pH 10.0 | Bacillus sp. (in: Bacteria) |
5 | 10 | recombinant deglycosylated ABA at 37°C, approx. 50% of maximal activity at pH 5.0, approx. 30% of maximal activity at pH 10.0 | Bacillus sp. (in: Bacteria) |