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Literature summary for 3.2.1.1 extracted from
- Characterization of active barley alpha-amylase 1 expressed and secreted by Saccharomyces cerevisiae (2002), J. Protein Chem., 20, 619-623.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
4,6-ethylidene-[G7]-p-nitrophenyl[G1]-alpha-D-maltoheptaoside | 37°C, pH 6.0 | Hordeum vulgare |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Hordeum vulgare | - |
maltooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Amy1, affinity chromatography on cyclohepta-amylase substituted epoxy-Sepharose 6B | Hordeum vulgare |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1900 | - |
recombinant Amy1, hydrolysis of soluble starch at 37°C and pH 6.0 | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside + H2O | - |
Hordeum vulgare | p-nitrophenol + 4,6-ethyliden-[G7]-alpha-D-maltoheptaoside | - |
? | |
| starch + H2O | - |
Hordeum vulgare | maltooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha amylase 1 | - |
Hordeum vulgare |
| AMY1 | - |
Hordeum vulgare |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 120 | - |
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside | 37°C, pH 6.0 | Hordeum vulgare | |
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Release 2023.1 (January 2023)
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