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Literature summary for 3.2.1.1 extracted from

  • Dey, S.; Agarwal, S.O.
    Characterization of a thermostable alpha-amylase from a thermophilic Streptomyces megasporus strain SD12 (1999), Indian J. Biochem. Biophys., 36, 150-157.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 23% loss of activity Streptomyces megasporus
Ag+ 1 mM AgCl2, 77% loss of activity Streptomyces megasporus
Ba2+ 1 mM BaCl2, 40% loss of activity Streptomyces megasporus
Co2+ 1 mM CoCl2, 65% loss of activity Streptomyces megasporus
Cu2+ 1 mM CuCl2, 28% loss of activity Streptomyces megasporus
EDTA 1 mM, 85% loss of activity Streptomyces megasporus
Fe2+ 1 mM FeCl2, 38% loss of activity Streptomyces megasporus
Hg2+ 1 mM HgCl2, 88% inhibition Streptomyces megasporus
Mg2+ 1 mM MgCl2, 30% loss of activity Streptomyces megasporus
Mn2+ 1 mM, 55% loss of activity Streptomyces megasporus
Pb2+ 1 mM, 50% loss of activity Streptomyces megasporus
PCMB 1 mM, 92% loss of activity Streptomyces megasporus
Zn2+ 1 mM ZnCl2, 45% loss of activity Streptomyces megasporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4.4 mg/ml for starch Streptomyces megasporus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces megasporus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 x * 97000, SDS-PAGE Streptomyces megasporus

Organism

Organism UniProt Comment Textmining
Streptomyces megasporus
-
-
-
Streptomyces megasporus SD12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces megasporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Streptomyces megasporus additional information major products are maltotriose and maltose ?
starch + H2O
-
 Streptomyces megasporus additional information maltose and traces of maltotriose ?
starch + H2O
-
 Streptomyces megasporus SD12 additional information major products are maltotriose and maltose ?
starch + H2O
-
 Streptomyces megasporus SD12 additional information maltose and traces of maltotriose ?

Subunits

Subunits Comment Organism
monomer x * 97000, SDS-PAGE Streptomyces megasporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces megasporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 45°C: about 50% of maximal activity, 75°C: about 60% of maximal activity Streptomyces megasporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 2 h Streptomyces megasporus
80
-
 half-life: 90 min Streptomyces megasporus
85
-
 half-life: 60 min Streptomyces megasporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3000
-
 starch calculated as low molecular weight products Streptomyces megasporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces megasporus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 60% of maximal activity, pH 7.5: about 50% of maximal activity Streptomyces megasporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 stable Streptomyces megasporus