Any feedback?
Literature summary for 3.2.1.1 extracted from
- Cloning, sequencing, characterization, and expression of an extracellular alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli and Bacillus subtilis (1997), J. Biol. Chem., 272, 16335-16342.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Bacillus subtilis | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slight | Pyrococcus furiosus |  |
| Cu2+ | 5 mM, complete inhibition | Pyrococcus furiosus | |
| Fe2+ | slight | Pyrococcus furiosus | |
| Ni2+ | slight inhibition | Pyrococcus furiosus | |
| Zn2+ | 5 mM, complete inhibition | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pyrococcus furiosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52875 | - |
x * 52875, extracellular enzyme, calculation from nucleotide sequence | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | O08452 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
| amylose + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
| maltodextran + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| maltodextrin + H2O | white dextrin | Pyrococcus furiosus | ? | - |
? | |
| starch + H2O | soluble starch | Pyrococcus furiosus | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52875, extracellular enzyme, calculation from nucleotide sequence | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Pyrococcus furiosus |
| 100 | 105 | - |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 130 | 70°C: about 55% of maximal activity, 130°C: about 65% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
6 h, 20% loss of activity | Pyrococcus furiosus |
| 115 | - |
3 h, 65% loss of activity | Pyrococcus furiosus |
| 120 | - |
1 h, 40% loss of activity | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | pH 3.5: about 70% of maximal activity, pH 7.5: about 40% of maximal activity | Pyrococcus furiosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
