Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 39% loss of activity of the membrane-bound enzyme | Bacillus subtilis | |
diisopropyl fluorophosphate | 1 mM, 34% inhibition of the membrane-bound enzyme | Bacillus subtilis | |
EDTA | 10 mM, 67% inhibition | Bacillus subtilis | |
EGTA | 10 mM, 53% inhibition of the membrane-bound enzyme | Bacillus subtilis | |
N-alpha-p-tosyl-L-lysine chloromethyl ketone | 1 mM, 22% loss of the activity of the membrane-bound enzyme | Bacillus subtilis | |
PMSF | 1 mM, 14% loss of activity of the membrane-bound enzyme | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
membrane | bound to, the enzyme is latent in membrane vesicles | Bacillus subtilis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 40 mM calcium acetate, enhances activity of membrane-bound enzyme to 268% of control | Bacillus subtilis | |
Mg2+ | 40 mM magnesium acetate, enhances activity of the membrane-bound enzyme to 216% of the control | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
extracellular enzyme, gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
Marburg strain | - |
Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | - |
Marburg strain | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | membrane-derived enzyme contains phospholipid, predominantly phosphatidylethanolamine | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
extracellular and membrane-bound enzyme | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, extracellular enzyme, SDS-PAGE | Bacillus subtilis |