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Literature summary for 3.2.1.1 extracted from

  • Melasniemi, H.
    Characterization of alpha-amylase and pullulanase activities of Clostridium thermohydrosulfuricum. Evidence for a novel thermostable amylase (1987), Biochem. J., 246, 193-197.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA reverses stimulation by Ca2+ Thermoanaerobacter thermohydrosulfuricus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermoanaerobacter thermohydrosulfuricus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Thermoanaerobacter thermohydrosulfuricus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter thermohydrosulfuricus
-
-
-
Thermoanaerobacter thermohydrosulfuricus E 101-69
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus panose + ?
-
?
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus E 101-69 panose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85 90
-
Thermoanaerobacter thermohydrosulfuricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 95 60°C: about 40% of maximal activity, 60°C: about 60% of maximal activity Thermoanaerobacter thermohydrosulfuricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
stabilizes at 85°C, fails to protect against inactivation at 95°C Thermoanaerobacter thermohydrosulfuricus
65
-
stable in absence of substrates and Ca2+ for at least 2 h Thermoanaerobacter thermohydrosulfuricus
85
-
30% loss of activity after 10 min, complete inactivation after 40 min, about 40% loss of activity after 2 h in presence of 2 mM Ca2+ Thermoanaerobacter thermohydrosulfuricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
at 60°C Thermoanaerobacter thermohydrosulfuricus
5.6
-
at 85°C Thermoanaerobacter thermohydrosulfuricus