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Literature summary for 3.13.2.1 extracted from

  • Czyrko, J.; Jaskolski, M.; Brzezinski, K.
    Crystal structure of S-adenosyl-L-homocysteine hydrolase from Cytophaga hutchinsonii, a case of combination of crystallographic and non-crystallographic symmetry (2018), Croat. Chem. Acta, 91, 153-162 .
No PubMed abstract available
Search for more literature for 3.13.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Cytophaga hutchinsonii
expression in Escherichia coli BL21 Cytophaga hutchinsonii

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in ternary complex with the oxidized form of the NAD+ cofactor and adenosine, hanging drop vapor diffusion method, using 15 % (w/v) PEG 8000 and 0.5 M Li2SO4 Cytophaga hutchinsonii
hanging-drop vapor-diffusion method, crystals are orthorhombic, space group P2(1)2(1)2 with unit cell parameters a = 96.28, b = 102.44, and c = 188.92 A Cytophaga hutchinsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0152
-
 S-adenosyl-L-homocysteine pH 7.5, 22°C Cytophaga hutchinsonii
0.0152
-
 S-adenosyl-L-homocysteine at pH 7.5 and 22°C Cytophaga hutchinsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ contains a sodium cation in close proximity of the active site Cytophaga hutchinsonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Cytophaga hutchinsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homocysteine + adenosine Cytophaga hutchinsonii
-
 S-adenosyl-L-homocysteine + H2O
-
 r
L-homocysteine + adenosine Cytophaga hutchinsonii ATCC 33406
-
 S-adenosyl-L-homocysteine + H2O
-
 r
S-adenosyl-L-homocysteine + H2O Cytophaga hutchinsonii
-
 L-homocysteine + adenosine
-
 ?
S-adenosyl-L-homocysteine + H2O Cytophaga hutchinsonii
-
 L-homocysteine + adenosine
-
 r
S-adenosyl-L-homocysteine + H2O Cytophaga hutchinsonii ATCC 33406
-
 L-homocysteine + adenosine
-
 ?
S-adenosyl-L-homocysteine + H2O Cytophaga hutchinsonii ATCC 33406
-
 L-homocysteine + adenosine
-
 r

Organism

Organism UniProt Comment Textmining
Cytophaga hutchinsonii A0A6N4SNR7
-
-
Cytophaga hutchinsonii ATCC 33406 A0A6N4SNR7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Cytophaga hutchinsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homocysteine + adenosine
-
 Cytophaga hutchinsonii S-adenosyl-L-homocysteine + H2O
-
 r
L-homocysteine + adenosine
-
 Cytophaga hutchinsonii ATCC 33406 S-adenosyl-L-homocysteine + H2O
-
 r
S-adenosyl-L-homocysteine + H2O
-
 Cytophaga hutchinsonii L-homocysteine + adenosine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Cytophaga hutchinsonii L-homocysteine + adenosine
-
 r
S-adenosyl-L-homocysteine + H2O
-
 Cytophaga hutchinsonii ATCC 33406 L-homocysteine + adenosine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Cytophaga hutchinsonii ATCC 33406 L-homocysteine + adenosine
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Cytophaga hutchinsonii
tetramer the asymmetric unit of the enzyme (ChSAHase) crystal contains four subunits of the enzyme. They do not form one functional tetrameric unit. They are assembled into two dimers, located at two different crystallographic twofold axes, whose operation recreates two tetramers that are related by non-crystallographic translation Cytophaga hutchinsonii

Synonyms

Synonyms Comment Organism
S-adenosyl-L-homocysteine hydrolase
-
 Cytophaga hutchinsonii
SAHase
-
 Cytophaga hutchinsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
 S-adenosyl-L-homocysteine pH 7.5, 22°C Cytophaga hutchinsonii
0.042
-
 S-adenosyl-L-homocysteine at pH 7.5 and 22°C Cytophaga hutchinsonii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cytophaga hutchinsonii