Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.13.2.1 extracted from

  • Khare, P.; Gupta, A.K.; Gajula, P.K.; Sunkari, K.Y.; Jaiswal, A.K.; Das, S.; Bajpai, P.; Chakraborty, T.K.; Dube, A.; Saxena, A.K.
    Identification of novel S-adenosyl-L-homocysteine hydrolase inhibitors through homology-model-based virtual screening, synthesis, and biological evaluation (2012), J. Chem. Inf. Model., 52, 777-791.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta cells Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-3-[(tert-butoxycarbonyl)(methyl)amino]-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]propanoate 63.62% inhibition at 0.2 mM Leishmania donovani
ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate 79.87% inhibition at 0.2 mM Leishmania donovani
ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-5-(6-amino-9H-purin-9-yl)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate 57.21% inhibition at 0.2 mM Leishmania donovani
ethyl 3-[(2R,3R,4R,5S)-5-[2-(acetylamino)-6-[(diphenylcarbamoyl)oxy]-9H-purin-9-yl]-4-(acetyloxy)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate 87.58% inhibition at 0.2 mM Leishmania donovani
ethyl 3-[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-[(tert-butoxycarbonyl)amino]-4-hydroxytetrahydrofuran-2-yl]propanoate 81.13% inhibition at 0.2 mM Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
x * 52000, SDS-PAGE Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Leishmania donovani
-
L-homocysteine + adenosine
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani D3K115 isolate 2001
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania donovani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
Leishmania donovani L-homocysteine + adenosine
-
?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
AdoHcyase
-
Leishmania donovani
S-adenosyl-L-homocysteine hydrolase
-
Leishmania donovani
S-AdoHcyase
-
Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Leishmania donovani

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00043
-
at pH 7.2 and 37°C Leishmania donovani ethyl 3-[(2R,3R,4R,5S)-5-[2-(acetylamino)-6-[(diphenylcarbamoyl)oxy]-9H-purin-9-yl]-4-(acetyloxy)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate
0.0796
-
at pH 7.2 and 37°C Leishmania donovani ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate
0.0954
-
at pH 7.2 and 37°C Leishmania donovani ethyl 3-[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-[(tert-butoxycarbonyl)amino]-4-hydroxytetrahydrofuran-2-yl]propanoate
0.144
-
at pH 7.2 and 37°C Leishmania donovani ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-3-[(tert-butoxycarbonyl)(methyl)amino]-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]propanoate
0.171
-
at pH 7.2 and 37°C Leishmania donovani ethyl 3-[(2R,3R,4R,5S)-4-(acetyloxy)-5-(6-amino-9H-purin-9-yl)-3-[(tert-butoxycarbonyl)(methyl)amino]tetrahydrofuran-2-yl]propanoate