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Literature summary for 3.13.2.1 extracted from

  • Wang, Y.; Kavran, J.M.; Chen, Z.; Karukurichi, K.R.; Leahy, D.J.; Cole, P.A.
    Regulation of S-adenosylhomocysteine hydrolase by lysine acetylation (2014), J. Biol. Chem., 289, 31361-31372.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.13.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 200 mM sodium formate, 5-20% (w/v) PEG 3350, and 100 mM BisTris, pH 6.0-6.5 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D293A the mutant shows reduced catalytic efficiency with S-adenosyl-L-homocysteine compared to the wild type enzyme and no reaction with L-homocysteine Homo sapiens
D422K inactive Homo sapiens
N27K the mutant shows reduced catalytic efficiency with S-adenosyl-L-homocysteine compared to the wild type enzyme and no reaction with L-homocysteine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0139
-
 S-adenosyl-L-homocysteine recombinant wild type enzyme, at pH 7.2 and 37°C Homo sapiens
0.0231
-
 S-adenosyl-L-homocysteine mutant enzyme N27K, at pH 7.2 and 37°C Homo sapiens
0.0334
-
 S-adenosyl-L-homocysteine mutant enzyme D293A, at pH 7.2 and 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homocysteine + adenosine + H2O Homo sapiens
-
 S-adenosyl-L-homocysteine
-
 r
S-adenosyl-L-homocysteine + H2O Homo sapiens
-
 L-homocysteine + adenosine
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P23526
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylation Lys401 and Lys408 acetylation of the enzyme changes hydrogen bonding patterns in its NAD+ binding regions and reduces its catalytic activity Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
chitin bead chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homocysteine + adenosine + H2O
-
 Homo sapiens S-adenosyl-L-homocysteine
-
 r
S-adenosyl-L-homocysteine + H2O
-
 Homo sapiens L-homocysteine + adenosine
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 48000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
S-adenosylhomocysteine hydrolase
-
 Homo sapiens
SAHH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 S-adenosyl-L-homocysteine mutant enzyme N27K, at pH 7.2 and 37°C Homo sapiens
0.56
-
 S-adenosyl-L-homocysteine mutant enzyme D293A, at pH 7.2 and 37°C Homo sapiens
0.79
-
 S-adenosyl-L-homocysteine recombinant wild type enzyme, at pH 7.2 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 S-adenosyl-L-homocysteine mutant enzyme D293A, at pH 7.2 and 37°C Homo sapiens
17
-
 S-adenosyl-L-homocysteine mutant enzyme N27K, at pH 7.2 and 37°C Homo sapiens
57
-
 S-adenosyl-L-homocysteine recombinant wild type enzyme, at pH 7.2 and 37°C Homo sapiens
2000
-
 L-homocysteine recombinant wild type enzyme, at pH 7.2 and 37°C Homo sapiens