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Literature summary for 3.13.2.1 extracted from

  • Qian, G.; Chen, C.; Zhou, R.; He, Y.; Shao, W.
    A thermostable S-adenosylhomocysteine hydrolase from Thermotoga maritima: properties and its application on S-adenosylhomocysteine production with enzymatic cofactor regeneration (2014), Enzyme Microb. Technol., 64-65, 33-37.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Thermotoga maritima
-
L-homocysteine + adenosine
-
?
S-adenosyl-L-homocysteine + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
L-homocysteine + adenosine
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima O51933
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 O51933
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized nickel-affinity column chromatography, and gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
Thermotoga maritima L-homocysteine + adenosine
-
?
S-adenosyl-L-homocysteine + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 L-homocysteine + adenosine
-
?

Synonyms

Synonyms Comment Organism
S-adenosylhomocysteine hydrolase
-
Thermotoga maritima
SAHase
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
in the presence of 1 mM NAD+ Thermotoga maritima
100
-
in the presence of 8 mM NAD+ Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in the presence of 1 mM NAD+ Thermotoga maritima
11.2
-
in the presence of 8 mM NAD+ Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermotoga maritima