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Literature summary for 3.13.2.1 extracted from

  • Lozada-Ramirez, J.D.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    Recombinant S-adenosylhomocysteine hydrolase from Thermotoga maritima: cloning, overexpression, characterization, and thermal purification studies (2013), Appl. Biochem. Biotechnol., 170, 639-653.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 gold cells Thermotoga maritima

General Stability

General Stability Organism
the enzyme is stable in the presence of urea 6 M when pre-incubated at room temperature for 48 h Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine
-
Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
S-adenosyl-L-homocysteine at pH 6.5 and 75°C Thermotoga maritima
0.049
-
adenosine at pH 6.5 and 75°C Thermotoga maritima
0.135
-
L-homocysteine at pH 6.5 and 75°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000, SDS-PAGE Thermotoga maritima
180000
-
gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homocysteine + adenosine Thermotoga maritima
-
S-adenosyl-L-homocysteine + H2O
-
r
L-homocysteine + adenosine Thermotoga maritima DSM 3109
-
S-adenosyl-L-homocysteine + H2O
-
r
S-adenosyl-L-homocysteine + H2O Thermotoga maritima
-
L-homocysteine + adenosine
-
r
S-adenosyl-L-homocysteine + H2O Thermotoga maritima DSM 3109
-
L-homocysteine + adenosine
-
r

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by thermal precipitation Thermotoga maritima

Storage Stability

Storage Stability Organism
-20°C, 50 mM phosphate buffer pH 6.5, at least 3 months, no loss of activity Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homocysteine + adenosine
-
Thermotoga maritima S-adenosyl-L-homocysteine + H2O
-
r
L-homocysteine + adenosine
-
Thermotoga maritima DSM 3109 S-adenosyl-L-homocysteine + H2O
-
r
S-adenosyl-L-homocysteine + H2O
-
Thermotoga maritima L-homocysteine + adenosine
-
r
S-adenosyl-L-homocysteine + H2O
-
Thermotoga maritima DSM 3109 L-homocysteine + adenosine
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 44000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
S-adenosyl-L-homocysteine hydrolase
-
Thermotoga maritima
S-adenosylhomocysteine hydrolase
-
Thermotoga maritima
SAHase
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 85 the enzyme shows more than 50% activity between 55 and 85°C Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 100 the apparent melting temperature is 87.6°C. After 10 min incubation at 80, 85, 90, 95, and 100°C the enzyme shows residual activity of about 97%, 70%, 20%, 8%, and 1%, respectively Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Thermotoga maritima

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.9
-
L-cysteine at pH 6.5 and 75°C Thermotoga maritima