Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 gold cells | Thermotoga maritima |
General Stability | Organism |
---|---|
the enzyme is stable in the presence of urea 6 M when pre-incubated at room temperature for 48 h | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
S-adenosyl-L-homocysteine | at pH 6.5 and 75°C | Thermotoga maritima | |
0.049 | - |
adenosine | at pH 6.5 and 75°C | Thermotoga maritima | |
0.135 | - |
L-homocysteine | at pH 6.5 and 75°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Thermotoga maritima |
180000 | - |
gel filtration | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homocysteine + adenosine | Thermotoga maritima | - |
S-adenosyl-L-homocysteine + H2O | - |
r | |
L-homocysteine + adenosine | Thermotoga maritima DSM 3109 | - |
S-adenosyl-L-homocysteine + H2O | - |
r | |
S-adenosyl-L-homocysteine + H2O | Thermotoga maritima | - |
L-homocysteine + adenosine | - |
r | |
S-adenosyl-L-homocysteine + H2O | Thermotoga maritima DSM 3109 | - |
L-homocysteine + adenosine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified by thermal precipitation | Thermotoga maritima |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer pH 6.5, at least 3 months, no loss of activity | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homocysteine + adenosine | - |
Thermotoga maritima | S-adenosyl-L-homocysteine + H2O | - |
r | |
L-homocysteine + adenosine | - |
Thermotoga maritima DSM 3109 | S-adenosyl-L-homocysteine + H2O | - |
r | |
S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima | L-homocysteine + adenosine | - |
r | |
S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima DSM 3109 | L-homocysteine + adenosine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 44000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
S-adenosyl-L-homocysteine hydrolase | - |
Thermotoga maritima |
S-adenosylhomocysteine hydrolase | - |
Thermotoga maritima |
SAHase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 85 | the enzyme shows more than 50% activity between 55 and 85°C | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | the apparent melting temperature is 87.6°C. After 10 min incubation at 80, 85, 90, 95, and 100°C the enzyme shows residual activity of about 97%, 70%, 20%, 8%, and 1%, respectively | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
L-cysteine | at pH 6.5 and 75°C | Thermotoga maritima |