Literature summary for 3.13.2.1 extracted from

He, M.; Zheng, Y.; Huang, C.H.; Qian, G.; Xiao, X.; Ko, T.P.; Shao, W.; Guo, R.T.
Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima (2014), Acta Crystallogr. Sect. F, 70, 1563-1565.

Search for more literature for 3.13.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.3 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, and 8% (w/v) polyethylene glycol 8000	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	Thermotoga maritima	-	L-homocysteine + adenosine	-	r
S-adenosyl-L-homocysteine + H2O	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	-	L-homocysteine + adenosine	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	O51933	-	-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	O51933	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-affinity column chromatography	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	-	Thermotoga maritima	L-homocysteine + adenosine	-	r
S-adenosyl-L-homocysteine + H2O	-	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	L-homocysteine + adenosine	-	r

Subunits

Subunits	Comment	Organism
homotetramer	x-ray crystallography	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
S-adenosyl-L-homocysteine hydrolase	-	Thermotoga maritima
SAHH	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
75	-	the enzyme exhibits extreme thermostability and retains 95% enzyme activity after incubation at 75°C for 2 h	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Thermotoga maritima

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Release 2023.1 (January 2023)